دورية أكاديمية
Apical and basolateral expression of aquaporin-1 in transfected MDCK and LLC-PK cells and functional evaluation of their transcellular osmotic water permeabilities.
| العنوان: | Apical and basolateral expression of aquaporin-1 in transfected MDCK and LLC-PK cells and functional evaluation of their transcellular osmotic water permeabilities. |
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| المؤلفون: | Deen PM; Department of Cell Physiology, University of Nijmegen, The Netherlands., Nielsen S, Bindels RJ, van Os CH |
| المصدر: | Pflugers Archiv : European journal of physiology [Pflugers Arch] 1997 Apr; Vol. 433 (6), pp. 780-7. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 0154720 Publication Model: Print Cited Medium: Print ISSN: 0031-6768 (Print) Linking ISSN: 00316768 NLM ISO Abbreviation: Pflugers Arch Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin, New York, Springer. |
| مواضيع طبية MeSH: | Aquaporins*, Cell Membrane Permeability/*physiology , Ion Channels/*biosynthesis, 1-Methyl-3-isobutylxanthine/pharmacology ; Animals ; Aquaporin 1 ; Blotting, Northern ; Blotting, Southern ; Cell Membrane Permeability/drug effects ; Cell Membrane Permeability/genetics ; Dogs ; Immunoblotting ; Ion Channels/genetics ; Kidney/cytology ; Kidney/drug effects ; Kidney/metabolism ; LLC-PK1 Cells ; Microscopy, Immunoelectron ; Osmolar Concentration ; Phosphodiesterase Inhibitors/pharmacology ; RNA, Messenger/biosynthesis ; Swine ; Tetradecanoylphorbol Acetate/pharmacology ; Transfection |
| مستخلص: | Aquaporin-1 is present in the apical and basolateral membranes in proximal tubules and descending limbs of Henlé's loop. In order ot be able to study the routing of Aquaporin-1 and the regulation of Aquaporin-1-mediated transcellular water flow, we stably transfected LLC-PK1 and MDCK-HRS cell lines with an Aquaporin-1 expression construct. LLC-PK1 clone 7 and MDCK clone K integrated two and one copies, respectively, which was reflected in the amount of Aquaporin-1 mRNA expressed in both clones. The Aquaporin-1 protein levels, however, were similar. In both clones, immuno-electronmicroscopy showed extensive labelling of Aquaporin-1 on the basolateral plasma membrane, endosomal vesicles and the apical plasma membrane, including the microvilli. To measure transcellular water permeation, a simple method was applied using phenol-red as a cell-impermeant marker of concentration. In contrast to the native cell lines, both clones revealed a high transcellular osmotic water permeability, which could not be influenced by forskolin add/3-isobutyl-1-methylxanthine (IBMX) or the phorbol ester 12-O-tetradecanoyl 13-acetate (TPA). After glutaraldehyde fixation, it was inhibitable by HgCl2. These results indicate that targeting of Aquaporin-1 to the apical and basolateral plasma membrane is independent of cell type and show for the first time that water flow through a cultured epithelium can be blocked by mercurial compounds. |
| المشرفين على المادة: | 0 (Aquaporins) 0 (Ion Channels) 0 (Phosphodiesterase Inhibitors) 0 (RNA, Messenger) 146410-94-8 (Aquaporin 1) NI40JAQ945 (Tetradecanoylphorbol Acetate) TBT296U68M (1-Methyl-3-isobutylxanthine) |
| تواريخ الأحداث: | Date Created: 19970401 Date Completed: 19970610 Latest Revision: 20131121 |
| رمز التحديث: | 20240513 |
| DOI: | 10.1007/s004240050345 |
| PMID: | 9049170 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0031-6768 |
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| DOI: | 10.1007/s004240050345 |