دورية أكاديمية
[Apparent wall shear rate and leukocyte-endothelium interaction in iris blood vessels in endotoxin-induced uveitis].
| العنوان: | [Apparent wall shear rate and leukocyte-endothelium interaction in iris blood vessels in endotoxin-induced uveitis]. |
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| عنوان ترانسليتريتد: | Scheinbare Wandscherrate und Leukozyten-Endothel-Interaktion in Irisgefässen bei Endotoxin-induzierter Uveitis. |
| المؤلفون: | Baatz H; Augenklinik der Charité, Humboldt-Universität, Berlin., Pleyer U, Hammer C, Thiel HJ |
| المصدر: | Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft [Ophthalmologe] 1996 Dec; Vol. 93 (6), pp. 749-53. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | German |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 9206148 Publication Model: Print Cited Medium: Print ISSN: 0941-293X (Print) Linking ISSN: 0941293X NLM ISO Abbreviation: Ophthalmologe Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin ; New York : Springer-Verlag, c1992- |
| مواضيع طبية MeSH: | Leukocyte Adherence Inhibition Test*, Bacterial Toxins/*immunology , Cell Adhesion Molecules/*physiology , Endothelium, Vascular/*immunology , Endotoxins/*immunology , Iris/*blood supply , Salmonella typhimurium/*immunology , Uveitis, Anterior/*immunology, Animals ; Blood Flow Velocity/physiology ; Female ; Microcirculation/physiology ; Rats ; Rats, Inbred Lew ; Venules |
| مستخلص: | Unlabelled: Inflammatory stimuli induce the expression of adhesion molecules on leukocytes and endothelial cells. The adhesive forces between leukocytes and the vascular endothelium are antagonized by the shear stress of the flowing blood. The aim of this study was to examine how differences in the apparent wall shear rate measured in iris venules with varying caliber affect leukocyte-endothelium interaction. Methods: The microvasculature of the iris in Lewis rats (n = 6) was examined using intravital fluorescence microscopy [2]. Blood flow was visualized with FITC-HES and leukocytes were stained with rhodamine 6G. Venules (n = 110) ranging in diameter from 20-60 microns were analysed with regard to the following parameters: Flux of leukocytes, velocity of leukocytes in the center stream, number of rolling and adherent leukocytes. The apparent wall shear rate was calculated from the center stream velocity and the vessel diameter. Animals were examined 4 h after administration of endotoxin from S. typhimurium (500 mg/kg body wt i.p.), when leukocyte-endothelium interaction is strongly enhanced. Results: The flux of leukocytes, the absolute number of rolling leukocytes and the velocity of leukocytes in the center stream was higher in larger than in smaller venules. The apparent wall shear rate decreased with increasing vessel diameter. Calculated values were 806 +/- 59 s-1 for vessels 20-30 microns in diameter and 483 +/- 34 s-1 for vessels of 50-60 microns (mean +/- SEM). The number of adherent leukocytes per mm2 endothelial surface and the rolling fraction did not show significant differences between vessels with varying caliber. Conclusion: Although the shear rate declined with increasing vessel diameter, we could not observe an increase in rolling and adherent leukocytes. The effects of vessel diameter on leukocyte adhesion to the vascular endothelium in postcapillary iris venules are minimal. |
| المشرفين على المادة: | 0 (Bacterial Toxins) 0 (Cell Adhesion Molecules) 0 (Endotoxins) 0 (salmonella toxin) |
| تواريخ الأحداث: | Date Created: 19961201 Date Completed: 19970328 Latest Revision: 20191024 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s003470050071 |
| PMID: | 9081537 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 0941-293X |
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| DOI: | 10.1007/s003470050071 |