دورية أكاديمية
Relative involvement of GPIb/IX-vWF axis and GPIIb/IIIa in thrombus growth at high shear rates in the guinea pig.
| العنوان: | Relative involvement of GPIb/IX-vWF axis and GPIIb/IIIa in thrombus growth at high shear rates in the guinea pig. |
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| المؤلفون: | André P; INSERM U353, Paris, France., Hainaud P, Bal dit Sollier C, Garfinkel LI, Caen JP, Drouet LO |
| المصدر: | Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 1997 May; Vol. 17 (5), pp. 919-24. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 9505803 Publication Model: Print Cited Medium: Print ISSN: 1079-5642 (Print) Linking ISSN: 10795642 NLM ISO Abbreviation: Arterioscler Thromb Vasc Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1998- : Baltimore, Md. : Lippincott Williams & Wilkins Original Publication: Dallas, TX : American Heart Association, c1995- |
| مواضيع طبية MeSH: | Blood Coagulation* , Hemorheology*, Blood Platelets/*physiology , Platelet Glycoprotein GPIb-IX Complex/*physiology , von Willebrand Factor/*physiology, Acetates/pharmacology ; Animals ; Anticoagulants/pharmacology ; Aurintricarboxylic Acid/pharmacology ; Guinea Pigs ; Male ; Peptide Fragments/pharmacology ; Platelet Adhesiveness ; Recombinant Proteins/pharmacology ; Tyrosine/analogs & derivatives ; Tyrosine/pharmacology |
| مستخلص: | The relative involvement of the glycoprotein (GP) Ib/IX-von Willebrand factor (vWF) axis and GPIIb/IIIa in thrombus growth at high shear rates was assessed and compared by testing the pharmacological effects of VCL, a recombinant GPIb-binding fragment of vWF (residues 504-728), aurintricarboxylic acid (ATA), which binds to the 509-695 disulfide loop of vWF, and lamifiban, a specific synthetic GPIIb/IIIa antagonist. In vivo, their effects were evaluated in guinea pig mesenteric arteries, in a model of a laser-induced cyclic thrombotic process, and ex vivo, at a shear rate of 1800 s(-1), in a capillary perfusion chamber model, in which collagen-adherent platelets are exposed to nonanticoagulated guinea pig blood. In vivo, VCL, ATA, and lamifiban administered 2 minutes after intimal injuries stopped thrombus growth, prevented the cyclic thrombotic process, and induced gradual thrombus dissolution. Ex vivo, at 1800 s(-1), collagen exposure to untreated blood for 2 minutes, 4 minutes, or two consecutive periods of 2 minutes each resulted in similar platelet adhesion, 56%, 59%, and 61%, respectively, with an average thrombus volume of 6, 19, and 17.5 microm3/microm2, respectively, without any fibrin formation. This indicated that the two consecutive perfusions did not affect the dynamic process of thrombus growth. When collagen-adherent platelets deposited after the first 2-minute perfusion were perfused for 2 minutes with VCL-, ATA-, or lamifiban-treated blood, thrombus growth was prevented and platelet adhesion remained unchanged, but fibrin formation increased on and around the predeposited platelets. These results suggest that both the GPIb/IX-vWF axis and GPIIb/IIIa are involved in in vivo platelet-to-platelet interactions at high shear rates in the guinea pig. |
| المشرفين على المادة: | 0 (Acetates) 0 (Anticoagulants) 0 (Peptide Fragments) 0 (Platelet Glycoprotein GPIb-IX Complex) 0 (Recombinant Proteins) 0 (von Willebrand Factor) 42HK56048U (Tyrosine) 4431-00-9 (Aurintricarboxylic Acid) 9XOE28082S (lamifiban) |
| تواريخ الأحداث: | Date Created: 19970501 Date Completed: 19970612 Latest Revision: 20190831 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1161/01.atv.17.5.919 |
| PMID: | 9157956 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1079-5642 |
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| DOI: | 10.1161/01.atv.17.5.919 |