دورية أكاديمية
أعرض في EDS

Heterogeneity of beta-adrenoceptors in guinea-pig brain: radioligand binding and cyclic nucleotide generation.

التفاصيل البيبلوغرافية
العنوان: Heterogeneity of beta-adrenoceptors in guinea-pig brain: radioligand binding and cyclic nucleotide generation.
المؤلفون: Neil KE; Department of Physiology and Pharmacology, University of Nottingham Medical School, Queen's Medical Centre, England., Hernández F, Kendall DA, Alexander SP
المصدر: Journal of neurochemistry [J Neurochem] 1997 Jun; Vol. 68 (6), pp. 2610-7.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley on behalf of the International Society for Neurochemistry Country of Publication: England NLM ID: 2985190R Publication Model: Print Cited Medium: Print ISSN: 0022-3042 (Print) Linking ISSN: 00223042 NLM ISO Abbreviation: J Neurochem Subsets: MEDLINE
أسماء مطبوعة: Publication: 2001- : Oxford, UK : Wiley on behalf of the International Society for Neurochemistry
Original Publication: New York : Raven Press
مواضيع طبية MeSH: Cerebellum/*metabolism , Cyclic AMP/*biosynthesis , Cyclic GMP/*biosynthesis , Receptors, Adrenergic, beta/*metabolism, Adenosine/analogs & derivatives ; Adenosine/pharmacology ; Adenosine-5'-(N-ethylcarboxamide) ; Adrenergic beta-Agonists/pharmacology ; Adrenergic beta-Antagonists/pharmacology ; Animals ; Atenolol/pharmacology ; Binding, Competitive/physiology ; Cerebellum/chemistry ; Female ; Guinea Pigs ; Inositol Phosphates/biosynthesis ; Iodine Radioisotopes ; Iodocyanopindolol ; Isoproterenol/pharmacology ; Male ; Pindolol/analogs & derivatives ; Pindolol/metabolism ; Pindolol/pharmacology ; Population ; Propanolamines/pharmacology ; Propranolol/pharmacology ; Radioligand Assay ; Signal Transduction/physiology ; Vasodilator Agents/pharmacology
مستخلص: In this report, we have examined the radioligand binding and second messenger signalling characteristics of beta-adrenoceptors in the guinea-pig brain. [125I]-Iodocyanopindolol ([125I]ICYP)-labelled sites in the cerebellum and cerebral cortex were of similar densities (Bmax 34 and 24 fmol x mg(-1)) and affinities (K(D) 20 and 55 pM), respectively. Analysis of competition for [125I]ICYP binding in the cerebellum was compatible with the presence of a beta2-adrenoceptor. In this tissue, isoprenaline evoked a cyclic AMP stimulation, and also potentiated cyclic GMP accumulations evoked in the presence of a nitric oxide donor, consistent with mediation via a beta2-adrenoceptor. The [125I]ICYP binding profile in the cerebral cortex did not comply with those previously described for beta-adrenoceptor subtypes, and isoprenaline failed to alter significantly cyclic AMP accumulation in the cerebral cortex, hippocampus, or neostriatum, even in the presence of forskolin or a phosphodiesterase inhibitor. Isoprenaline was also without effect on cyclic GMP accumulation or phosphoinositide turnover in the cerebral cortex. These results suggest that the guinea-pig cerebellum expresses a functional beta2-adrenoceptor coupled to cyclic AMP generation, and potentiation of cyclic GMP accumulation. However, the guinea-pig cerebral cortex displays binding sites that exhibit beta-adrenoceptor-like pharmacology but fail to show functional coupling to cyclic AMP, cyclic GMP, or phosphoinositide signalling systems.
المشرفين على المادة: 0 (Adrenergic beta-Agonists)
0 (Adrenergic beta-Antagonists)
0 (Inositol Phosphates)
0 (Iodine Radioisotopes)
0 (Propanolamines)
0 (Receptors, Adrenergic, beta)
0 (Vasodilator Agents)
35920-39-9 (Adenosine-5'-(N-ethylcarboxamide))
46OL1UC10R (ICI 118551)
50VV3VW0TI (Atenolol)
83498-72-0 (Iodocyanopindolol)
9Y8NXQ24VQ (Propranolol)
BJ4HF6IU1D (Pindolol)
E0399OZS9N (Cyclic AMP)
H2D2X058MU (Cyclic GMP)
K72T3FS567 (Adenosine)
L628TT009W (Isoproterenol)
تواريخ الأحداث: Date Created: 19970601 Date Completed: 19970617 Latest Revision: 20190630
رمز التحديث: 20231215
DOI: 10.1046/j.1471-4159.1997.68062610.x
PMID: 9166759
قاعدة البيانات: MEDLINE
الوصف
تدمد:0022-3042
DOI:10.1046/j.1471-4159.1997.68062610.x