دورية أكاديمية
Delayed response of QM- and DA/DAPI-fluorescence in C-heterochromatin of the small Japanese field mouse, Apodemus argenteus.
| العنوان: | Delayed response of QM- and DA/DAPI-fluorescence in C-heterochromatin of the small Japanese field mouse, Apodemus argenteus. |
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| المؤلفون: | Obara Y; Department of Biology, Faculty of Science, Hirosaki University, Aomori, Japan., Sasaki S, Igarashi Y |
| المصدر: | Zoological science [Zoolog Sci] 1997 Feb; Vol. 14 (1), pp. 57-64. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Zoological Society of Japan Country of Publication: Japan NLM ID: 8702287 Publication Model: Print Cited Medium: Print ISSN: 0289-0003 (Print) Linking ISSN: 02890003 NLM ISO Abbreviation: Zoolog Sci |
| أسماء مطبوعة: | Original Publication: Tokyo, Japan : Zoological Society of Japan, c1984- |
| مواضيع طبية MeSH: | Fluorescent Dyes* , Indoles* , Quinacrine Mustard*, Heterochromatin/*chemistry , Muridae/*genetics, Animals ; Chromosome Banding ; Fluorescence ; Heterochromatin/genetics ; Mice |
| مستخلص: | The small Japanese field mouse Apodemus argenteus has the diploid chromosome number of 46, carrying rather large centromeric C-heterochromatin in most of the 44 autosomes and a large amount of C-heterochromatin in the sex chromosomes: the largest subtelocentric X was heterochromatic in almost two-fifth (whole short arm and proximal part of the long arm) of its entire length and the medium-sized acrocentric Y was totally heterochromatic. The C-heterochromatin (C-positive) areas, other than those of the Y and smallest three pairs, had a unique property of "delayed QM-fluorescence", which has not been reported to-date, showing dull QM-fluorescence immediately after exposure to blue light (BL), but gradually turning to bright fluorescence in a few minutes. The fluorescence intensity gradually decreased after attaining its peak, and finally became extinct. A similar pattern of fluorescence was also obtained in DA/DAPI-stained-X chromosome C-heterochromatin, but not in autosomal C-heterochromatin. No such dull-to-bright transition of QM-fluorescence could be obtained by CMA staining, for which the C-positive areas were apparently negative even after overexposure to BL. These facts indicate that the C-positive areas of A. argenteus showing dull-to-bright transition of QM-fluorescence contain A-T rich DNA. The delayed QM-fluorescence was found only in A. argenteus, in thirteen mammalian species so-far examined. Furthermore, this unique property of QM-fluorescence could be artificially altered to non-delayed ordinary type of fluorescence by sequentially pretreating the fixed chromosomes with hydrochloride and barium hydroxide solutions. The cytological implication of the delayed fluorescence in the C-heterochromatin of A. argenteus is briefly discussed. |
| المشرفين على المادة: | 0 (Fluorescent Dyes) 0 (Heterochromatin) 0 (Indoles) 4213-45-0 (Quinacrine Mustard) 47165-04-8 (DAPI) |
| تواريخ الأحداث: | Date Created: 19970201 Date Completed: 19970807 Latest Revision: 20061115 |
| رمز التحديث: | 20240628 |
| DOI: | 10.2108/zsj.14.57 |
| PMID: | 9200979 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0289-0003 |
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| DOI: | 10.2108/zsj.14.57 |