دورية أكاديمية
Restoration of thymopoiesis in pT alpha-/- mice by anti-CD3epsilon antibody treatment or with transgenes encoding activated Lck or tailless pT alpha.
| العنوان: | Restoration of thymopoiesis in pT alpha-/- mice by anti-CD3epsilon antibody treatment or with transgenes encoding activated Lck or tailless pT alpha. |
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| المؤلفون: | Fehling HJ; Basel Institute for Immunology, Switzerland., Iritani BM, Krotkova A, Forbush KA, Laplace C, Perlmutter RM, von Boehmer H |
| المصدر: | Immunity [Immunity] 1997 Jun; Vol. 6 (6), pp. 703-14. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 9432918 Publication Model: Print Cited Medium: Print ISSN: 1074-7613 (Print) Linking ISSN: 10747613 NLM ISO Abbreviation: Immunity Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, MA : Cell Press Original Publication: Cambridge, Mass. : Cell Press, c1994- |
| مواضيع طبية MeSH: | CD3 Complex/*physiology , Receptors, Antigen, T-Cell, alpha-beta/*chemistry , T-Lymphocytes/*cytology , src-Family Kinases/*physiology, Animals ; Cell Differentiation ; Cytoplasm ; Lymphocyte Specific Protein Tyrosine Kinase p56(lck) ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mice, Transgenic ; Receptors, Antigen, T-Cell, alpha-beta/genetics ; Sequence Deletion ; Signal Transduction ; Structure-Activity Relationship ; Thymus Gland/cytology ; Transgenes |
| مستخلص: | Mice deficient for the pre-TCR alpha (pT alpha) chain cannot form a pre-T cell receptor (TCR) and exhibit a severe defect in early T cell development, characterized by lack of "beta selection" and impaired generation of double-positive (DP) thymocytes. Here, we demonstrate that intraperitoneal injection of CD3epsilon-specific antibodies into pT alpha-/- x RAG-/- mice or introduction of an activated p56(lck) transgene in pT alpha-/- mice fully restores the number of DP thymocytes, and that expression of a transgenic pT alpha chain lacking its cytoplasmic portion can overcome all developmental defects associated with pT alpha deficiency. These results allow a better definition of the role of pT alpha in pre-TCR signal transduction and provide conclusive evidence that the cytoplasmic tail of pT alpha is not essential for pre-TCR signaling. |
| معلومات مُعتمدة: | K08 AI001445-04 United States AI NIAID NIH HHS; K08 AI001445-05 United States AI NIAID NIH HHS; K08 AI001445-06 United States AI NIAID NIH HHS |
| المشرفين على المادة: | 0 (CD3 Complex) 0 (Receptors, Antigen, T-Cell, alpha-beta) EC 2.7.10.2 (Lymphocyte Specific Protein Tyrosine Kinase p56(lck)) EC 2.7.10.2 (src-Family Kinases) |
| تواريخ الأحداث: | Date Created: 19970601 Date Completed: 19970728 Latest Revision: 20190914 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/s1074-7613(00)80446-x |
| PMID: | 9208843 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1074-7613 |
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| DOI: | 10.1016/s1074-7613(00)80446-x |