دورية أكاديمية
[A new type of IP3-sensitive highly selective calcium channels of low conductance in the plasma membrane of carcinoma A 431 cells].
| العنوان: | [A new type of IP3-sensitive highly selective calcium channels of low conductance in the plasma membrane of carcinoma A 431 cells]. |
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| عنوان ترانسليتريتد: | Novyĭ tip IP3-chuvstvitel'nykh vysokoselektivnykh kal'tsievykh kanalov nizkoĭ provodimosti v plazmaticheskoĭ membrane kletok kartsinomy A 431. |
| المؤلفون: | Kiselev KI, Mamin AG, Semenova SB, Mozhaeva GN |
| المصدر: | Tsitologiia [Tsitologiia] 1997; Vol. 39 (6), pp. 395-408. |
| نوع المنشور: | English Abstract; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Rossiiskaia Akademiia Nauk Country of Publication: Russia (Federation) NLM ID: 0417363 Publication Model: Print Cited Medium: Print ISSN: 0041-3771 (Print) Linking ISSN: 00413771 NLM ISO Abbreviation: Tsitologiia Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Moskva : Rossiiskaia Akademiia Nauk Original Publication: Moskva, Akademiia nauk SSSR. |
| مواضيع طبية MeSH: | Calcium Channel Agonists/*pharmacology , Calcium Channels/*drug effects , Inositol 1,4,5-Trisphosphate/*pharmacology, Barium/metabolism ; Bradykinin/pharmacology ; Calcium/metabolism ; Calcium Channels/physiology ; Carcinoma, Squamous Cell ; Cell Membrane/drug effects ; Cell Membrane/physiology ; Dose-Response Relationship, Drug ; Electric Conductivity ; Heparin/pharmacology ; Humans ; Membrane Potentials/drug effects ; Membrane Potentials/physiology ; Patch-Clamp Techniques ; Stimulation, Chemical ; Tumor Cells, Cultured ; Uridine Triphosphate/pharmacology |
| مستخلص: | Application of 0.2-15.0 mM inositol 1,4,5-trisphosphate (IP3) to excised plasma membrane patches from A 431 cells induced activity of low conductance channels that were highly selective for Ca2+ and Ba2+. With 100 mM Ca2+ or 105 mM Ba2+, the channel conductance was 1.1 pS for the minor conductance substrate. The dose response curve gave an apparent binding constant of 0.18 microM IP3. The channel open probability displayed a strong potential dependence: it decreased markedly upon depolarization, and the half-maximum value was achieved at 73 mV. The dependence of channel activity on the intracellular free Ca2+ concentration was bell shaped, but markedly different from that reported for endoplasmic IP3 receptor. The same channels were detected in intact cells upon application of calcium mobilizing reagents. The activity induced in cell attached mode disappeared after patch excision but could be resumed by application of IP3. The data obtained argue for IP3 acting directly on plasma membrane Ca2+ channels. |
| المشرفين على المادة: | 0 (Calcium Channel Agonists) 0 (Calcium Channels) 24GP945V5T (Barium) 85166-31-0 (Inositol 1,4,5-Trisphosphate) 9005-49-6 (Heparin) S8TIM42R2W (Bradykinin) SY7Q814VUP (Calcium) UT0S826Z60 (Uridine Triphosphate) |
| تواريخ الأحداث: | Date Created: 19970101 Date Completed: 19971120 Latest Revision: 20131121 |
| رمز التحديث: | 20231215 |
| PMID: | 9381558 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0041-3771 |
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