دورية أكاديمية
Expression of two alternative sigma factors of Synechococcus sp. strain PCC 7002 is modulated by carbon and nitrogen stress.
| العنوان: | Expression of two alternative sigma factors of Synechococcus sp. strain PCC 7002 is modulated by carbon and nitrogen stress. |
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| المؤلفون: | Caslake LF, Gruber TM; Department of Biochemistry and Molecular Biology and Center for Biomolecular Structure and Function, The Pennsylvania State University, University Park,PA 16802,USA., Bryant DA; Department of Biochemistry and Molecular Biology and Center for Biomolecular Structure and Function, The Pennsylvania State University, University Park,PA 16802,USA. |
| المصدر: | Microbiology (Reading, England) [Microbiology (Reading)] 1997 Dec; Vol. 143 ( Pt 12), pp. 3807-3818. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 9430468 Publication Model: Print Cited Medium: Print ISSN: 1350-0872 (Print) Linking ISSN: 13500872 NLM ISO Abbreviation: Microbiology (Reading) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: Reading, U.K. : Society for General Microbiology, c1994- |
| مواضيع طبية MeSH: | DNA-Binding Proteins* , Gene Expression Regulation, Bacterial* , Transcription, Genetic*, Bacterial Proteins/*biosynthesis , Cyanobacteria/*metabolism , Sigma Factor/*biosynthesis, Amino Acid Sequence ; Bacterial Proteins/chemistry ; Carbon/metabolism ; Cloning, Molecular ; Cyanobacteria/genetics ; DNA-Directed RNA Polymerases/biosynthesis ; DNA-Directed RNA Polymerases/chemistry ; Genes, Bacterial ; Heat-Shock Proteins/biosynthesis ; Molecular Sequence Data ; Nitrogen/metabolism ; RNA, Messenger/biosynthesis ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/chemistry ; Restriction Mapping ; Sequence Alignment ; Sequence Homology, Amino Acid ; Sigma Factor/chemistry |
| مستخلص: | The sigB and sigC genes, encoding two alternative sigma factors of the unicellular marine cyanobacterium Synechococcus sp. PCC 7002, were cloned and characterized. Strains in which the sigB and sigC genes were insertionally inactivated were viable under standard laboratory conditions, indicating that SigB and SigC are group 2 sigma factors. Starvation for either nitrogen or carbon caused an increase in sigB mRNA levels. Transcripts for the sigC gene initially increased but then decreased during nitrogen and carbon starvation. The SigC protein could not be identified in cyanobacterial extracts using antisera to Synechococcus sp. PCC 7002 SigA or RpoD from Bacillus subtilis. The ratio of the principal vegetative sigma factor, SigA, to SigB decreased during either nitrogen starvation or carbon starvation, and the levels of SigB also increased in the sigC mutant strain. These results imply that SigB and SigC play roles in modifying transcription in response to changes in carbon and nitrogen availability in this cyanobacterium. |
| معلومات مُعتمدة: | GM31625 United States GM NIGMS NIH HHS |
| سلسلة جزيئية: | GENBANK U82435; U82436 |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (DNA-Binding Proteins) 0 (Heat-Shock Proteins) 0 (RNA, Messenger) 0 (Recombinant Proteins) 0 (SigB protein, Bacteria) 0 (Sigma Factor) 0 (sigC protein, Bacteria) 7440-44-0 (Carbon) EC 2.7.7.6 (DNA-Directed RNA Polymerases) N762921K75 (Nitrogen) |
| تواريخ الأحداث: | Date Created: 19980109 Date Completed: 19980217 Latest Revision: 20210303 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1099/00221287-143-12-3807 |
| PMID: | 9421905 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1350-0872 |
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| DOI: | 10.1099/00221287-143-12-3807 |