دورية أكاديمية
Activity of cyclic AMP phosphodiesterases and adenylyl cyclase in peripheral nerve after crush and permanent transection injuries.
| العنوان: | Activity of cyclic AMP phosphodiesterases and adenylyl cyclase in peripheral nerve after crush and permanent transection injuries. |
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| المؤلفون: | Walikonis RS; Department of Neurology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA., Poduslo JF |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 1998 Apr 10; Vol. 273 (15), pp. 9070-7. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print Cited Medium: Print ISSN: 0021-9258 (Print) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Nerve Crush* , Nerve Regeneration* , Phosphoric Diester Hydrolases*, 3',5'-Cyclic-AMP Phosphodiesterases/*biosynthesis , 3',5'-Cyclic-GMP Phosphodiesterases/*biosynthesis , Adenylyl Cyclases/*biosynthesis , Myelin Sheath/*physiology , Sciatic Nerve/*injuries , Sciatic Nerve/*physiology , Transcription, Genetic/*drug effects, 1-Methyl-3-isobutylxanthine/pharmacology ; Animals ; Cyclic AMP/metabolism ; Cyclic Nucleotide Phosphodiesterases, Type 1 ; Cyclic Nucleotide Phosphodiesterases, Type 4 ; Gene Expression Regulation, Enzymologic/drug effects ; Male ; Pyrrolidinones/pharmacology ; Quinolones/pharmacology ; RNA, Messenger/biosynthesis ; Rats ; Rats, Sprague-Dawley ; Reference Values ; Rolipram ; S100 Proteins/biosynthesis ; Time Factors ; Xanthines/pharmacology |
| مستخلص: | Recent studies demonstrate that cAMP levels are tightly controlled during demyelination and remyelination in Schwann cells as cAMP decreases to 8-10% of normal following both sciatic nerve crush or permanent transection injury and only begins to increase in the crushed nerve after remyelination (Poduslo, J. F., Walikonis, R. S., Domec, M., Berg, C. T., and Holtz-Heppelmann, C. J. (1995) J. Neurochem. 65, 149-159). To investigate the mechanisms responsible for this change in cAMP levels, cAMP phosphodiesterase (PDE) and adenylyl cyclase activities were determined before and after sciatic nerve injury. Basal cAMP PDE activity in soluble endoneurial homogenates of normal nerve was 34.9 +/- 1.9 pmol/mg of protein/min (chi +/- S.E.; n = 10). This activity increased about 3-fold within 6 days following both injuries. Basal PDE activity remained elevated in the transected nerve, but declined to 70 pmol/mg of protein/min in the crushed nerve at 21 and 35 days following injury. Isozyme-specific inhibitors and stimulators were used to identify the PDE families in the sciatic nerve. The low Km cAMP-specific (PDE4) and the Ca2+/calmodulin-stimulated (PDE1) families were found to predominate in assays using endoneurial homogenates. The PDE4 inhibitor rolipram also increased cAMP levels significantly after incubation of endoneurial tissue with various isozyme-specific inhibitors, indicating that PDE4 plays a major role in determining cAMP levels. PDE4 mRNA was localized by in situ hybridization to cells identified as Schwann cells by colabeling of S100, a Schwann cell specific protein. Adenylyl cyclase activity declined following injury, from 3.7 pmol/mg of protein/min in normal nerve to 0.70 pmol/mg/min by 7 days following injury. Both decreased synthesis and increased degradation contribute, therefore, to the reduced levels of cAMP following peripheral nerve injury and are likely critical to the process of Wallerian degeneration. |
| المشرفين على المادة: | 0 (Pyrrolidinones) 0 (Quinolones) 0 (RNA, Messenger) 0 (S100 Proteins) 0 (Xanthines) 04B949KO6F (denbufylline) 45S5605Q18 (cilostamide) E0399OZS9N (Cyclic AMP) EC 3.1.4.- (Phosphoric Diester Hydrolases) EC 3.1.4.17 (3',5'-Cyclic-AMP Phosphodiesterases) EC 3.1.4.17 (Cyclic Nucleotide Phosphodiesterases, Type 1) EC 3.1.4.17 (Cyclic Nucleotide Phosphodiesterases, Type 4) EC 3.1.4.35 (3',5'-Cyclic-GMP Phosphodiesterases) EC 4.6.1.1 (Adenylyl Cyclases) K676NL63N7 (Rolipram) TBT296U68M (1-Methyl-3-isobutylxanthine) |
| تواريخ الأحداث: | Date Created: 19980516 Date Completed: 19980514 Latest Revision: 20210209 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1074/jbc.273.15.9070 |
| PMID: | 9535895 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0021-9258 |
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| DOI: | 10.1074/jbc.273.15.9070 |