دورية أكاديمية
A chromosomally based tod-luxCDABE whole-cell reporter for benzene, toluene, ethybenzene, and xylene (BTEX) sensing.
| العنوان: | A chromosomally based tod-luxCDABE whole-cell reporter for benzene, toluene, ethybenzene, and xylene (BTEX) sensing. |
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| المؤلفون: | Applegate BM; Department of Microbiology, University of Tennessee, Knoxville 37996-1605, USA., Kehrmeyer SR, Sayler GS |
| المصدر: | Applied and environmental microbiology [Appl Environ Microbiol] 1998 Jul; Vol. 64 (7), pp. 2730-5. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print Cited Medium: Print ISSN: 0099-2240 (Print) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Gene Expression Regulation, Bacterial*, DNA, Bacterial/*drug effects , Hydrocarbons, Aromatic/*pharmacology , Pseudomonas putida/*drug effects, Benzene/pharmacology ; DNA Transposable Elements/genetics ; DNA, Bacterial/genetics ; Gene Expression Regulation ; Plasmids/genetics ; Pseudomonas putida/genetics ; Toluene/pharmacology ; Xylenes/pharmacology |
| مستخلص: | A tod-luxCDABE fusion was constructed and introduced into the chromosome of Pseudomonas putida F1, yielding the strain TVA8. This strain was used to examine the induction of the tod operon when exposed to benzene, toluene, ethylbenzene, and xylene (BTEX) compounds and aqueous solutions of JP-4 jet fuel constituents. Since this system contained the complete lux cassette (luxCDABE), bacterial bioluminescence in response to putative chemical inducers of the tod operon was measured on-line in whole cells without added aldehyde substrate. There was an increasing response to toluene concentrations from 30 micrograms/liter to 50 mg/liter, which began to saturate at higher concentrations. The detection limit was 30 micrograms/liter. There was a significant light response to benzene, m- and p-xylenes, phenol, and water-soluble JP-4 jet fuel components, but there was no bioluminescence response upon exposure to o-xylene. The transposon insertion was stable and had no negative effect on cell growth. |
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| المشرفين على المادة: | 0 (DNA Transposable Elements) 0 (DNA, Bacterial) 0 (Hydrocarbons, Aromatic) 0 (Xylenes) 3FPU23BG52 (Toluene) J64922108F (Benzene) |
| تواريخ الأحداث: | Date Created: 19980702 Date Completed: 19980811 Latest Revision: 20200724 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC106455 |
| DOI: | 10.1128/AEM.64.7.2730-2735.1998 |
| PMID: | 9647859 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0099-2240 |
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| DOI: | 10.1128/AEM.64.7.2730-2735.1998 |