دورية أكاديمية
Influence of hairpins on template reannealing at trinucleotide repeat duplexes: a model for slipped DNA.
| العنوان: | Influence of hairpins on template reannealing at trinucleotide repeat duplexes: a model for slipped DNA. |
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| المؤلفون: | Gacy AM; Department of Biochemistry and Molecular Biology, Mayo Foundation and Mayo Graduate School, Rochester, Minnesota 55905, USA., McMurray CT |
| المصدر: | Biochemistry [Biochemistry] 1998 Jun 30; Vol. 37 (26), pp. 9426-34. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370623 Publication Model: Print Cited Medium: Print ISSN: 0006-2960 (Print) Linking ISSN: 00062960 NLM ISO Abbreviation: Biochemistry Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Nucleic Acid Conformation* , Trinucleotide Repeats*, DNA/*chemistry , Nucleic Acid Heteroduplexes/*chemistry, Base Sequence ; DNA/chemical synthesis ; DNA, Complementary/chemistry ; DNA, Single-Stranded/chemistry ; Electrophoresis, Polyacrylamide Gel ; Models, Molecular ; Molecular Sequence Data ; Nucleic Acid Heteroduplexes/chemical synthesis ; Oligonucleotides/chemical synthesis ; Templates, Genetic ; Thermodynamics |
| مستخلص: | Hairpin stabilization of polymerase slippage has been proposed as part of the mechanism for large-scale expansion of CG-rich (CNG, where N = A, T, G, or C) trinucleotide repeats. However, hairpin formation does not entirely account for why long repeats but not short repeats or palindromes expand. Using ultraviolet spectroscopic methods, we examine the thermodynamic and kinetic properties of repeating trinucleotides to evaluate their behavior at a slippage site. We find that CNG trinucleotide repeats associated with expansion form stable hairpins whether they are short (with as few as 10 repeats) or long. However, long repeating stretches exist as single strands up to 2 orders of magnitude longer than sequences with either short repeats or random DNA. Thus, long hairpins have long lifetimes even in the presence of their complementary strands and inhibit duplex reannealing at a slippage site. The kinetic properties explain why expansion occurs with high frequency at long repeats but not at short repeats or palindromes. |
| معلومات مُعتمدة: | DK 43694-01A2 United States DK NIDDK NIH HHS |
| المشرفين على المادة: | 0 (DNA, Complementary) 0 (DNA, Single-Stranded) 0 (Nucleic Acid Heteroduplexes) 0 (Oligonucleotides) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 19980702 Date Completed: 19980723 Latest Revision: 20071114 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1021/bi980157s |
| PMID: | 9649325 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 0006-2960 |
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| DOI: | 10.1021/bi980157s |