دورية أكاديمية
Sequence and functional characterization of RNase P RNA from the chl alb containing cyanobacterium Prochlorothrix hollandica.
العنوان: | Sequence and functional characterization of RNase P RNA from the chl alb containing cyanobacterium Prochlorothrix hollandica. |
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المؤلفون: | Fingerhut C; Institut für Biochemie, Bayerische Julius-Maximilians-Universität, Biozentrum, Würzburg, Germany., Schön A |
المصدر: | FEBS letters [FEBS Lett] 1998 May 29; Vol. 428 (3), pp. 161-4. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 0155157 Publication Model: Print Cited Medium: Print ISSN: 0014-5793 (Print) Linking ISSN: 00145793 NLM ISO Abbreviation: FEBS Lett Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd. Original Publication: Amsterdam, North-Holland on behalf of the Federation of European Biochemical Societies. |
مواضيع طبية MeSH: | Nucleic Acid Conformation*, Endoribonucleases/*chemistry , Endoribonucleases/*metabolism , Prochlorothrix/*metabolism , RNA, Bacterial/*chemistry , RNA, Bacterial/*metabolism , RNA, Catalytic/*chemistry , RNA, Catalytic/*metabolism, Base Sequence ; Cloning, Molecular ; Kinetics ; Models, Molecular ; Molecular Sequence Data ; Mutagenesis ; Polymerase Chain Reaction ; Prochlorothrix/genetics ; RNA Precursors/chemistry ; RNA Precursors/genetics ; RNA Precursors/metabolism ; RNA, Transfer, Tyr/chemistry ; RNA, Transfer, Tyr/genetics ; RNA, Transfer, Tyr/metabolism ; Ribonuclease P ; Sequence Deletion |
مستخلص: | Only a few complete sequences and very limited functional data are available for the catalytic RNA component of cyanobacterial RNase P. The RNase P RNA from the chl alb containing cyanobacterium Prochlorothrix hollandica belongs to a rarely found structural subtype with an extended P15/16 domain. We have established conditions for optimal in vitro ribozyme activity, and determined the kinetic parameters for cleavage of pre-tRNA(Tyr). Analysis of pre-tRNA mutants revealed that the T-stem sequence only plays a modulating role, whereas the CCA end is essential for efficient product formation. |
سلسلة جزيئية: | GENBANK Y16812 |
المشرفين على المادة: | 0 (RNA Precursors) 0 (RNA, Bacterial) 0 (RNA, Catalytic) 0 (RNA, Transfer, Tyr) 0 (pre-tRNA(Tyr)) EC 3.1.- (Endoribonucleases) EC 3.1.26.5 (Ribonuclease P) |
تواريخ الأحداث: | Date Created: 19980708 Date Completed: 19980727 Latest Revision: 20190621 |
رمز التحديث: | 20221213 |
DOI: | 10.1016/s0014-5793(98)00519-5 |
PMID: | 9654127 |
قاعدة البيانات: | MEDLINE |
تدمد: | 0014-5793 |
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DOI: | 10.1016/s0014-5793(98)00519-5 |