دورية أكاديمية
Western blot detection of infectious bursal disease virus infection.
| العنوان: | Western blot detection of infectious bursal disease virus infection. |
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| المؤلفون: | Pereira SR; Departamento de Virologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Brasil., Travassos CE, Huguenim A, Guimarães AC, Silva AG, Guimarães MA |
| المصدر: | Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas [Braz J Med Biol Res] 1998 May; Vol. 31 (5), pp. 671-4. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Associação Brasileira de Divulgação Científica Country of Publication: Brazil NLM ID: 8112917 Publication Model: Print Cited Medium: Print ISSN: 0100-879X (Print) Linking ISSN: 0100879X NLM ISO Abbreviation: Braz J Med Biol Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [SP, Brasil : Associação Brasileira de Divulgação Científica, 1981- |
| مواضيع طبية MeSH: | Birnaviridae Infections/*diagnosis , Chickens/*virology , Infectious bursal disease virus/*isolation & purification , Poultry Diseases/*diagnosis, Animals ; Antibodies, Viral/isolation & purification ; Antigens, Viral/isolation & purification ; Blotting, Western ; Infectious bursal disease virus/immunology ; Viral Matrix Proteins/isolation & purification |
| مستخلص: | In order to evaluate the use of a Western blot methodology for the diagnosis of infectious bursal disease virus (IBDV) infection, chickens were experimentally infected with IBDV strains and tested for the presence of viral antigens and antibodies by a blocking Western blot test (bWB). The viral proteins obtained from the bursa of Fabricius (BF) were transferred to a nitrocellulose membrane after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the chicken sera obtained by heart puncture were used for the detection of these proteins. In order to eliminate nonspecific reactions, we used a rabbit anti-chicken serum (blocking tool). By the use of the bWB test, two distinct viral proteins of 43-kDa (VP2) and 32-kDa (VP3) were detected. We suggest the use of this methodology for the detection of IBDV infection in animals suspected of having IBDV reinfection and a chronic subclinical form of the disease. With the use of the rabbit anti-chicken sera for blocking, this method is practical, sensitive and less time consuming. |
| Number of References: | 20 |
| المشرفين على المادة: | 0 (Antibodies, Viral) 0 (Antigens, Viral) 0 (Viral Matrix Proteins) |
| تواريخ الأحداث: | Date Created: 19980812 Date Completed: 19981020 Latest Revision: 20190516 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1590/s0100-879x1998000500011 |
| PMID: | 9698773 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0100-879X |
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| DOI: | 10.1590/s0100-879x1998000500011 |