دورية أكاديمية
Presence of germline and full-length IgA RNA transcripts among peritoneal B-1 cells.
| العنوان: | Presence of germline and full-length IgA RNA transcripts among peritoneal B-1 cells. |
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| المؤلفون: | de Waard R; Department of Histology and Cell Biology, University of Groningen, The Netherlands., Dammers PM, Tung JW, Kantor AB, Wilshire JA, Bos NA, Herzenberg LA, Kroese FG |
| المصدر: | Developmental immunology [Dev Immunol] 1998; Vol. 6 (1-2), pp. 81-7. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9200624 Publication Model: Print Cited Medium: Print ISSN: 1044-6672 (Print) Linking ISSN: 10267905 NLM ISO Abbreviation: Dev Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2002- : London : Taylor & Francis Original Publication: Chur [Switzerland] ; New York : Harwood Academic, 1990- |
| مواضيع طبية MeSH: | B-Lymphocytes/*metabolism , Immunoglobulin A/*genetics , Peritoneal Cavity/*cytology , RNA, Messenger/*analysis, Animals ; Female ; Male ; Mice ; Mice, Inbred BALB C ; Rats |
| مستخلص: | Next to conventional B cells (or B-2 cells), peritoneal B-1 cells have been shown to contribute significantly to the production of IgA-secreting plasma cells in the gut. Evidence for this was mainly based on studies comprising manipulated animals, including lethally X-irradiated and transgenic mice. To examine the ability of peritoneal B-1 cells from untreated mice to switch actively to IgA in vivo, we performed RT-PCR analysis on FACS-sorted peritoneal B-cell subsets from untreated BALB/c mice in order to examine the presence of germline C alpha mRNA and mature C alpha mRNA transcripts. Germline C alpha and mature C alpha transcripts were readily detectable in peritoneal B-1 cells (defined as IgMbright/IgDdull), but not, or very little, in peritoneal B-2 cells (defined as IgMdull/IgDbright). Moreover, by subdividing the B-1-cell population in CD5+ B-1a cells and CD5- B-1b cells, it was shown that in vivo expression of germline C alpha and mature C alpha transcripts was largely restricted to the B-1b-cell lineage. These results indicate that peritoneal B-1 cells indeed are capable to switch to IgA under normal physiological conditions and hereby further support the view that B-1 cells contribute significantly to the mucosal IgA response, albeit this function appears to be restricted to the B-1b-cell subset. |
| المشرفين على المادة: | 0 (Immunoglobulin A) 0 (RNA, Messenger) |
| تواريخ الأحداث: | Date Created: 19980826 Date Completed: 19981022 Latest Revision: 20191102 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC2275998 |
| DOI: | 10.1155/1998/37576 |
| PMID: | 9716908 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1044-6672 |
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| DOI: | 10.1155/1998/37576 |