دورية أكاديمية
أعرض في EDS

Role of autocrine stimulation for the effects of cyclic AMP on protein and lipid phosphorylation in platelets activated by particles.

التفاصيل البيبلوغرافية
العنوان: Role of autocrine stimulation for the effects of cyclic AMP on protein and lipid phosphorylation in platelets activated by particles.
المؤلفون: Ryningen A; Department of Biochemistry and Molecular Biology, University of Bergen, Norway. anita.ryningen@pki.uib.no, Holmsen H
المصدر: FEBS letters [FEBS Lett] 1998 Oct 09; Vol. 436 (3), pp. 335-8.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 0155157 Publication Model: Print Cited Medium: Print ISSN: 0014-5793 (Print) Linking ISSN: 00145793 NLM ISO Abbreviation: FEBS Lett Subsets: MEDLINE
أسماء مطبوعة: Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd.
Original Publication: Amsterdam, North-Holland on behalf of the Federation of European Biochemical Societies.
مواضيع طبية MeSH: Phosphoproteins* , Platelet Activation*/drug effects, Blood Platelets/*metabolism , Blood Proteins/*metabolism , Colforsin/*pharmacology , Cyclic AMP/*blood , Fat Emulsions, Intravenous/*pharmacology , Lipids/*blood , Metrizoic Acid/*analogs & derivatives, Adenosine Diphosphate/blood ; Adenosine Triphosphate/blood ; Blood Platelets/drug effects ; Contrast Media/pharmacology ; Cytoplasmic Granules/drug effects ; Cytoplasmic Granules/metabolism ; Humans ; In Vitro Techniques ; Lysosomes/drug effects ; Lysosomes/metabolism ; Metrizoic Acid/pharmacology ; Phosphates/blood ; Phospholipids/blood ; Phosphorylation ; Phosphotyrosine/blood ; Protein Kinase C/blood ; Serum Albumin
مستخلص: We have compared responses in platelets stimulated with the particulate materials, Intralipid (liposome-suspension) and a potential contrast medium IEEC (1'-(ethyloxycarbonyloxy)-ethyl-5-acetyl-amino-3-(N-methyl-acetyla mino)-2,4,6-triiodo-benzenecarboxylate coated with human serum albumin), with and without forskolin and inhibitors of autocrine stimulation (IAS: an ADP-removing system of creatine phosphate/creatine phosphokinase; RGDS to prevent fibrinogen/fibronectin binding to GPIIb/IIIa; SQ 29.548 as a TXA2 receptor antagonist; cyproheptadine as a serotonin receptor antagonist; BN 52021 as a platelet-activating factor receptor antagonist). The pattern of tyrosine-phosphorylated proteins, phosphorylation of initial lipids and phosphorylation of pleckstrin (P47) were used as markers for early signal transducing responses, while secretion of ADP+ATP and beta-N-acetyl-glycosaminidase were used as final responses. Intralipid showed no platelet activation except for some weak tyrosine protein phosphorylation that was inhibited by elevated cAMP. IEEC induced strong platelet activation that was partly inhibited by increased levels of cAMP and IAS. The inhibition of elevated cAMP seemed to be due to removal of the G protein-mediated activation from secreted autocrine stimulators either by IAS or forskolin. The remaining activity is a pure effect from IEEC which is not affected by elevated cAMP.
المشرفين على المادة: 0 ((1'-ethyloxycarbonyloxy)ethyl 5-acetylamino-3-(N-methylacetylamino)-2,4,6-triiodobenzenecarboxylate)
0 (Blood Proteins)
0 (Contrast Media)
0 (Fat Emulsions, Intravenous)
0 (Lipids)
0 (Phosphates)
0 (Phospholipids)
0 (Phosphoproteins)
0 (Serum Albumin)
0 (platelet protein P47)
1F7A44V6OU (Colforsin)
21820-51-9 (Phosphotyrosine)
61D2G4IYVH (Adenosine Diphosphate)
8L70Q75FXE (Adenosine Triphosphate)
CM1N99QR1M (Metrizoic Acid)
E0399OZS9N (Cyclic AMP)
EC 2.7.11.13 (Protein Kinase C)
تواريخ الأحداث: Date Created: 19981104 Date Completed: 19981123 Latest Revision: 20190621
رمز التحديث: 20221213
DOI: 10.1016/s0014-5793(98)01154-5
PMID: 9801143
قاعدة البيانات: MEDLINE
الوصف
تدمد:0014-5793
DOI:10.1016/s0014-5793(98)01154-5