دورية أكاديمية
Expression of p210 and p190 BCR-ABL due to alternative splicing in chronic myelogenous leukaemia.
| العنوان: | Expression of p210 and p190 BCR-ABL due to alternative splicing in chronic myelogenous leukaemia. |
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| المؤلفون: | Lichty BD; Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario Cancer Institute, Princess Margaret Hospital, Canada., Keating A, Callum J, Yee K, Croxford R, Corpus G, Nwachukwu B, Kim P, Guo J, Kamel-Reid S |
| المصدر: | British journal of haematology [Br J Haematol] 1998 Dec; Vol. 103 (3), pp. 711-5. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 0372544 Publication Model: Print Cited Medium: Print ISSN: 0007-1048 (Print) Linking ISSN: 00071048 NLM ISO Abbreviation: Br J Haematol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Wiley-Blackwell Original Publication: Oxford : Blackwell Scientific Publications |
| مواضيع طبية MeSH: | Alternative Splicing/*genetics , Fusion Proteins, bcr-abl/*genetics , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics, Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; Gene Expression ; Humans ; Middle Aged ; RNA, Neoplasm/genetics ; Reverse Transcriptase Polymerase Chain Reaction/methods |
| مستخلص: | The hallmark of chronic myelogenous leukaemia (CML) is the presence of the Philadelphia chromosome and its resultant fusion message, BCR-ABL, and fusion protein, p210. Patients with CML in blast crisis, or with Philadelphia positive acute lymphoblastic leukaemia (ALL), can have a smaller BCR-ABL fusion transcript possessing only the first exon of BCR fused to ABL. This smaller transcript encodes a 190 kD protein which is more strongly transforming than the p210 protein derived from the larger CML-associated transcript. We performed RT-PCR on samples from CML patients in chronic phase to determine the frequency and mechanism of p190 and p210 co-expression and to see if this correlated with clinical indices. We examined the peripheral blood or marrow of 67 patients with CML and found that 35 of them expressed both transcripts whereas the remainder expressed the p210-encoding transcript exclusively. Additional PCR products of an intermediate size were also frequently detected and have been isolated and sequenced. Data from two of these products indicate that they are the result of alternative splicing and include variable combinations of BCR exons. We believe that the expression of the p190-encoding transcript in the chronic phase of CML is also due to alternative splicing. A comparison of patients co-expressing the p190- and p210-encoding transcripts with those patients who expressed only the p210-encoding transcript detected significantly higher white blood cell (WBC) counts and blast cell counts at time of testing as well as significantly higher white blood cell counts at diagnosis. |
| المشرفين على المادة: | 0 (RNA, Neoplasm) EC 2.7.10.2 (Fusion Proteins, bcr-abl) |
| تواريخ الأحداث: | Date Created: 19981219 Date Completed: 19990127 Latest Revision: 20190705 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1046/j.1365-2141.1998.01033.x |
| PMID: | 9858221 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0007-1048 |
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| DOI: | 10.1046/j.1365-2141.1998.01033.x |