التفاصيل البيبلوغرافية
| العنوان: |
ARGONAUTE 6 bridges transposable element m RNA-derived si RNAs to the establishment of DNA methylation. |
| المؤلفون: |
McCue, Andrea D, Panda, Kaushik, Nuthikattu, Saivageethi, Choudury, Sarah G, Thomas, Erica N, Slotkin, R Keith |
| المصدر: |
EMBO Journal; Jan2015, Vol. 34 Issue 1, p20-35, 16p |
| مصطلحات موضوعية: |
TRANSPOSONS, MESSENGER RNA, SMALL interfering RNA, DNA methylation, GENETIC mutation, CHROMATIN |
| مستخلص: |
Transposable elements ( TEs) generate mutations and chromosomal instability when active. To repress TE activity, eukaryotic cells evolved mechanisms to both degrade TE mRNAs into small interfering RNAs (si RNAs) and modify TE chromatin to epigenetically inhibit transcription. Since the populations of small RNAs that participate in TE post-transcriptional regulation differ from those that establish RNA-directed DNA methylation ( Rd DM), the mechanism through which transcriptionally active TEs transition from post-transcriptional RNAi regulation to chromatin level control has remained unclear. We have identified the molecular mechanism of a plant pathway that functions to direct DNA methylation to transcriptionally active TEs. We demonstrated that 21-22 nucleotide (nt) si RNA degradation products from the RNAi of TE mRNAs are directly incorporated into the ARGONAUTE 6 ( AGO6) protein and direct AGO6 to TE chromatin to guide its function in Rd DM. We find that this pathway functions in reproductive precursor cells to primarily target long centromeric high-copy transcriptionally active TEs for Rd DM prior to gametogenesis. This study provides a direct mechanism that bridges the gap between the post-transcriptional regulation of TEs and the establishment of TE epigenetic silencing. [ABSTRACT FROM AUTHOR] |
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