التفاصيل البيبلوغرافية
| العنوان: |
Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells. |
| المؤلفون: |
Ferrara, Maria Antonietta, Caprio, Giuseppe Di, Managò, Stefano, De Angelis, Annalisa, Sirleto, Luigi, Coppola, Giuseppe, De Luca, Anna Chiara |
| المصدر: |
Biosensors (2079-6374); 2015, Vol. 5 Issue 2, p141-157, 17p |
| مصطلحات موضوعية: |
SPERMATOZOA analysis, ARTIFICIAL insemination of cattle, RAMAN spectroscopy, CELL imaging, CELL morphology, DIGITAL holographic microscopy |
| مستخلص: |
A full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order to study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) and Raman spectroscopy (RS). DH presents new opportunities for studying morphological aspect of cells and tissues non-invasively, quantitatively and without the need for staining or tagging, while RS is a very specific technique allowing the biochemical analysis of cellular components with a spatial resolution in the sub-micrometer range. In this paper, morphological and biochemical bovine sperm cell alterations were studied using these techniques. In addition, a complementary DH and RS study was performed to identify X- and Y-chromosome-bearing sperm cells. We demonstrate that the two techniques together are a powerful and highly efficient tool elucidating some important criterions for sperm morphological selection and sex-identification, overcoming many of the limitations associated with existing protocols. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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