التفاصيل البيبلوغرافية
| العنوان: |
基于 EDS 的鱼类耳石微化学分析实验条件选择. (Chinese) |
| Alternate Title: |
Experiment Condition Selection and Research of Fish Otolith Microchemistry Analysis Based on EDS. (English) |
| المؤلفون: |
GUO Hong-yi, ZHANG Xu-guang, SHAO Lu, YIN Xi-ming |
| المصدر: |
Research & Exploration in Laboratory; May2015, Vol. 34 Issue 5, p24-26, 3p |
| Abstract (English): |
This paper studies the application of spectrometer in fish otolith microchemistry analysis, and explores a set of experiment conditions for quantitative analysis of fish otolith standard samples. For electron microprobe analysis the otoliths were ground into uniform thickness chips, then dried at 60°C constant-temperature oven for 6-8 hours, and finally gold coated in a high-vacuum evaporator. In order to ensure the accuracy of experimental results, experiment parameters should be determined in accordance with otolith size, experimental objective for key composition of fish otolith and instrument performance. Accelerating voltage must be higher than X-ray critical excitation voltage of all element properties measured, which is more than 2 times and less than 10 times of critical excitation voltage Uk in general cases. The optimal working distance is 20 mm, with live time of over 200 s and deadtime between 10%-15% in single scan. Focusing parameters should be determined on line with otolith sample size, with full considerations to a short measurement time and sufficient spatial resolution. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
究能谱仪在鱼类耳石微化学分析中的应用, 探索一套适用于鱼类耳石标 准样品定量分析实验条件. 结果表明, 样品表面粗糙会对特征X 射线产生几何效 应， 使得分析强度降低, 造成实验结果误差.耳石样本最适用烘干干燥法, 其操作 过程为: 耳石放入干净培养狐后, 在恒温烤箱 60°C 烘干6 ~ 8 h. 样品干燥后，表面 喷金待用.为获得精确的分析结果, 实验参数应根据样品大小, 实验目的所需要关 注的组成元素和仪器性能来选择. 加速电压1 一般为临界激发电压U k 的 2 〜10 倍.最适工作距离为 20 mm, 单点担描活时间为 200s 以上, 死时间 10% 〜 15%. 聚焦参数应该根据耳石样品不同的大小选择, 尽可能兼顾缩短测量时间和保证足 够的空间分辨率。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
