التفاصيل البيبلوغرافية
| العنوان: |
Induction of interleukin (IL)-1β, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans. |
| المؤلفون: |
Trindade, S. C., Olczak, T., Gomes-Filho, I. S., Moura-Costa, L. F., Cerqueira, E. M. M., Galdino-Neto, M., Alves, H., Carvalho-Filho, P. C., Xavier, M. T., Meyer, R. |
| المصدر: |
Journal of Periodontal Research; Feb2012, Vol. 47 Issue 1, p27-32, 6p |
| مستخلص: |
Trindade SC, Olczak T, Gomes-Filho IS, Moura-Costa LF, Cerqueira EMM, Galdino-Neto M, Alves H, Carvalho-Filho PC, Xavier MT, Meyer R. Induction of interleukin (IL)-1β, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans. J Periodont Res 2012; 47: 27-32. © 2011 John Wiley & Sons A/S Background and Objective: Porphyromonas gingivalis, an anaerobic gram-negative bacterium, is associated with chronic periodontitis. This study was undertaken to evaluate the production of interleukin (IL)-1β, IL-8 and IL-10 by human peripheral blood mononuclear cells (PBMC) stimulated with P. gingivalis antigens and to assess the levels of serum immunoglobulin (Ig)G, IgA and IgG subclasses raised against P. gingivalis HmuY protein. Material and Methods: PBMC from patients with chronic periodontitis (CP) and from nonperiodontitis (NP) control subjects were stimulated with P. gingivalis antigens, and the cytokine levels in the culture supernatants were determined by ELISA. The specificity of serum antibodies raised against HmuY was analyzed by Western blotting and by ELISA. Results: Compared with the NP controls, the CP patients produced higher levels of total serum IgG and IgG1 specific for P. gingivalis HmuY. No differences were found between CP and NP groups in the production of IL-1β and IL-8 by PBMC stimulated with total P. gingivalis antigens. Only P. gingivalis lipopolysaccharide (LPS) induced higher levels of IL-10 in the CP group. Higher levels of IL-1β and IL-10 were induced by HmuY than by other antigens derived from the wild-type P. gingivalis strains. In contrast, total antigens derived from the hmuY-deletion mutant strain induced the production of significantly higher levels of IL-8 and significantly lower levels of IL-1β. Conclusion: Our data suggest that P. gingivalis HmuY may be considered an immunogenic protein associated with host-pathogen interactions. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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