التفاصيل البيبلوغرافية
العنوان: |
Girdin regulates the migration and invasion of glioma cells via the PI3K-Akt signaling pathway. |
المؤلفون: |
WEIMIN NI, YAN FANG, LEI TONG, ZHAOXUE TONG, FUXIN YI, JIANWU QIU, RUI WANG, XIAOJIE TONG |
المصدر: |
Molecular Medicine Reports; 2015, Vol. 12 Issue 4, p5086-5092, 7p |
مصطلحات موضوعية: |
GLIOMAS, PROTEIN kinases, ACTIN, CELL migration, GENE expression, CELL proliferation |
مستخلص: |
Girdin, an actin-binding protein, is associated with cell migration and is expressed at high levels in glioma cells. However, the association between girdin and the development of glioma remains to be elucidated. In the present study, short-hairpin RNA technology was used to silence the gene expression of girdin. The effects of girdin silencing on glioma cell proliferation, migration and invasion were then assessed using a cell viability assay, wound-healing assay, transwell invasion assay, reverse transcription-quantitative polymerase chain reaction, western blot analysis and gelatin zymography. The results suggested that girdin silencing inhibited the proliferation, migration and invasion of glioma cells. In addition, the expression levels and activity of matrix metalloproteinase (MMP)-2 and MMP-9 were also affected by girdin silencing. Further mechanistic investigation indicated that girdin may regulate glioma cell migration and invasion through the phosphatidylinositol-3-kinase/protein kinase B (PI3K-Akt) signaling pathway. Therefore, the results of the present study provide a theoretical foundation for the development of anticancer drugs. [ABSTRACT FROM AUTHOR] |
|
Copyright of Molecular Medicine Reports is the property of Spandidos Publications UK Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
قاعدة البيانات: |
Complementary Index |