التفاصيل البيبلوغرافية
| العنوان: |
Enhanced anti-inflammatory effects of DHA and quercetin in lipopolysaccharide-induced RAW264.7 macrophages by inhibiting NF-κB and MAPK activation. |
| المؤلفون: |
TIAN-LEI SI, QI LIU, YU-FEI REN, HUI LI, XIAO-YUN XU, ER-HU LI, SI-YI PAN, JIU-LIANG ZHANG, KE-XING WANG |
| المصدر: |
Molecular Medicine Reports; 2016, Vol. 14 Issue 1, p499-508, 10p |
| مصطلحات موضوعية: |
DOCOSAHEXAENOIC acid, QUERCETIN, ANTI-inflammatory agents, LIPOPOLYSACCHARIDES, MESSENGER RNA, CYCLOOXYGENASE 2, POLYMERASE chain reaction, ENZYME-linked immunosorbent assay |
| مستخلص: |
The aim of the present study was to investigate the anti-inflammatory effects of docosahexaenoic acid (DHA) + quercetin (QE) used in combination. DHA and QE are natural compounds derived from various foods and have been demonstrated to exert anti-inflammatory effects The protein mRNA expression involved in the nuclear factor (NF)-κB and mitogen-activated protein kinase (MAPK) signalling pathway was analyzed by western blot analysis and reverse transcription-polymerase chain reaction methods respectively, other cytokines were detected by an enzyme-linked immunosorbent assay kit. The results of the present study demonstrated that combined treatment of lipopolysaccharide (LPS)-stimulated RAW264.7 cells with DHA + QE decreased the levels of pro-inflammatory mediators to a greater extent than QE or DHA alone. Additionally, DHA + QE synergistically suppressed nitric oxide, prostaglandin E2 and cyclooxygenase-2 levels. Molecular-level studies indicated that the DHA + QE combination can significantly inhibit the mRNA expression of NF-κB subunits p50 and p65, extracellular signal-regulated kinase (ERK) 1/2 and c-JUN N-terminal kinase (JNK) 1/2, which suggests that the NF-κB signalling pathway is involved in the synergistic effects observed. Furthermore, western blot analysis demonstrated that DHA + QE synergistically inhibit the phosphorylation of p50, p65, ERK1/2 and JNK1/2. This finding indicates that the enhanced anti-inflammatory effects of the combined compounds are achieved by suppressing NF-κB and MAPK signalling in LPS-stimulated RAW264.7 cells. The results of the present study suggest that DHA and QE in combination may be utilized as potent anti-inflammatory compounds, with potential preventative or palliative effects on obesity, atherosclerosis and cardiovascular diseases. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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