التفاصيل البيبلوغرافية
| العنوان: |
β-Cell death is decreased in women with gestational diabetes mellitus. |
| المؤلفون: |
Kenna, Lauren A., Olsen, John A., Spelios, Michael G., Radin, Michael S., Akirav, Eitan M. |
| المصدر: |
Diabetology & Metabolic Syndrome; 8/24/2016, Vol. 8, p1-6, 6p, 1 Diagram, 2 Charts, 1 Graph |
| مصطلحات موضوعية: |
GESTATIONAL diabetes, PREGNANCY complications, MATERNAL health, INSULIN resistance, BIOMARKERS, POSTNATAL care, DIAGNOSIS |
| مستخلص: |
Background: Gestational diabetes mellitus (GDM) affects approximately 7-17 % of all pregnancies and has been recognized as a significant risk factor to neonatal and maternal health. Postpartum, GDM significantly increases the likelihood of developing type 2 diabetes (T2D). While it is well established that insulin resistance and impaired β-cell function contribute to GDM development, the role of active β-cell loss remains unknown. Differentially methylated circulating free DNA (cfDNA) is a minimally invasive biomarker of β-cell loss in type 1 diabetes mellitus. Here we use cfDNA to examine the levels of β-cell death in women with GDM. Methods: Second to third-trimester pregnant women with GDM were compared with women with normal pregnancy (PRG), women at postpartum (PP), and non-pregnant (NP) women. Fasting glucose levels, insulin, and C-peptide levels were measured. Serum samples were collected and cfDNA purified and bisulfite treated. Methylationsensitive probes capable of differentiating between β-cell-derived DNA (demethylated) and non-β-cell-derived DNA (methylated) were used to measure the presence of β-cell loss in the blood. Results: GDM was associated with elevated fasting glucose levels (GDM = 185.9 ± 5.0 mg/dL) and reduced fasting insulin and c-peptide levels when compared with NP group. Interestingly, β-cell derived insulin DNA levels were significantly lower in women with GDM when compared with PRG, NP, and PP groups (demethylation index: PRG = 7.74 × 10-3 ± 3.09 × 10-3, GDM = 1.01 × 10-3 ± 5.86 × 10-4, p < 0.04; NP = 4.53 × 10-3 ± 1.62 × 10-3, PP = 3.24 × 10-3 ± 1.78 × 10-3). Conclusions: These results demonstrate that β-cell death is reduced in women with GDM. This reduction is associated with impaired insulin production and hyperglycemia, suggesting that β-cell death does not contribute to GDM during the 2nd and 3rd trimester of pregnancy. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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