التفاصيل البيبلوغرافية
| العنوان: |
Activity-based protein profiling of hydrolytic enzymes induced by gibberellic acid in isolated aleurone layers of malting barley. |
| المؤلفون: |
Daneri-Castro, Sergio N., Chandrasekar, Balakumaran, Grosse-Holz, Friederike M., Hoorn, Renier A. L., Roberts, Thomas H. |
| المصدر: |
FEBS Letters; Sep2016, Vol. 590 Issue 17, p2956-2962, 7p |
| مصطلحات موضوعية: |
HYDROLASES, GIBBERELLIC acid, ALEURONE layer, MALTING, BARLEY enzymes, GERMINATION |
| مستخلص: |
During barley germination, the aleurone layer secretes most of the enzymes required to degrade the endosperm, many of which are yet to be characterized. We used activity-based protein profiling ( ABPP) to detect a range of active enzymes extracted from aleurone layers isolated from grains of a commercial malting barley variety incubated with or without gibberellic acid ( GA). Enzymes found to be induced by GA were putative aleurains, cathepsin-B-like proteases and serine hydrolases. By using an inhibitory sugar panel, a specific active retaining β-glycosidase in the barley aleurone was identified as a putative xylanase. Our results show that ABPP can be used rapidly to identify a variety of active enzyme isoforms in cereal aleurone without the need for enzyme purification. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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