التفاصيل البيبلوغرافية
| العنوان: |
The antibacterial activity and mechanism of ginkgolic acid C15:1. |
| المؤلفون: |
Zhebin Hua, Caie Wu, Gongjian Fan, Zhenxing Tang, Fuliang Cao |
| المصدر: |
BMC Biotechnology; 1/14/2017, Vol. 17, p1-12, 12p |
| مصطلحات موضوعية: |
ANTIBACTERIAL agents, BACTERIAL growth, GREEN fluorescent protein, BACTERIAL proteins, GRAM-negative bacteria |
| مستخلص: |
Background: The present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (GA) C15:1 monomer using green fluorescent protein (GFP)-labeled bacteria strains. Results: GA presented significant antibacterial activity against Gram-positive bacteria but generally did not affect the growth of Gram-negative bacteria. The studies of the antibacterial mechanism indicated that large amounts of GA (C15:1) could penetrate GFP-labeled Bacillus amyloliquefaciens in a short period of time, and as a result, led to the quenching of GFP in bacteria. In vitro results demonstrated that GA (C15:1) could inhibit the activity of multiple proteins including DNA polymerase. In vivo results showed that GA (C15:1) could significantly inhibit the biosynthesis of DNA, RNA and B. amyloliquefaciens proteins. Conclusion: We speculated that GA (C15:1) achieved its antibacterial effect through inhibiting the protein activity of B. amyloliquefaciens. GA (C15:1) could not penetrate Gram-negative bacteria in large amounts, and the lipid soluble components in the bacterial cell wall could intercept GA (C15:1), which was one of the primary reasons that GA (C15:1) did not have a significant antibacterial effect on Gram-negative bacteria. [ABSTRACT FROM AUTHOR] |
|
Copyright of BMC Biotechnology is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Find this article in full text from Springer Verlag. 
Full Text Finder