التفاصيل البيبلوغرافية
| العنوان: |
Cloning and Functional Analysis of Xylem Specific Promoter Deletion Fragment. |
| المؤلفون: |
Yajing LUO, Liang WANG, Kejiu DU, Shuang ZHANG |
| المصدر: |
Agricultural Biotechnology (2164-4993); Dec2016, Vol. 5 Issue 6, p1-10, 4p |
| مصطلحات موضوعية: |
PLANT cloning, XYLEM, DELETION mutation, TRANSCRIPTION factors, AGROBACTERIUM |
| مستخلص: |
Xylem-specific promoter could regulate efficient expression of foreign genes in xylem. Deletion analysis method was applied to obtain 5' deletion promoter fragments MU2 andMU4 with different distances from transcription start site of xylem-specific promoter MDCesAP through PCR procedure. Then CaMV35S promoter in plant expression vector pBI121 was replaced by amplified fragments. The recombinant plasmids fused with corresponding gene segments and GUS reporter gene were obtained and transformed into Agrobacterium. The results of the tobacco transient transformation test showed that MU2 andMU4 had the promoter function, and both of their activity were significantly higher than that of CaMV35S promoter, and they had the characteristics of xylem tissue specificity as well. Research on xylem-specific promoter structure and function could lay a foundation for the determination of necessary components to the xylem tissue specificity and cis-acting elements with induction activity, so as to enable the regulation of efficient expression of exogenous genes in specific time and specific tissues in plants through these cis-acting elements. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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