التفاصيل البيبلوغرافية
| العنوان: |
Tongxinluo Inhibits Renal Fibrosis in Diabetic Nephropathy: Involvement of the Suppression of Intercellular Transfer of TGF-β1-Containing Exosomes from GECs to GMCs. |
| المؤلفون: |
Xiao-Ming Wu, Yan-Bin Gao, Li-Ping Xu, Da-Wei Zou, Zhi-Yao Zhu, Xiao-Lei Wang, Wei-Jie Yao, Liang-Tao Luo, Yu Tong, Nian-Xiu Tian, Zhe-Ji Han, Wan-Yu Dang |
| المصدر: |
American Journal of Chinese Medicine; 2017, Vol. 45 Issue 5, p1075-1092, 18p, 6 Graphs |
| مصطلحات موضوعية: |
PHYTOTHERAPY, ANALYSIS of variance, ANIMAL experimentation, CELL culture, DIABETIC nephropathies, ELECTRON microscopy, ENZYME-linked immunosorbent assay, IMMUNOHISTOCHEMISTRY, MICE, POLYMERASE chain reaction, RESEARCH funding, STATISTICS, TRANSFORMING growth factors-beta, WESTERN immunoblotting, DATA analysis, FIBROSIS, DATA analysis software, EXOSOMES, IN vivo studies |
| مستخلص: |
Glomerular mesangial cells (GMCs) activation is implicated in the pathogenesis of diabetic nephropathy (DN). Our previous study revealed that high glucose (HG)-treated glomerular endothelial cells (GECs) produce an increased number of TGF-ß1-containing exosomes to activate GMCs through the TGF-ß1/Smad3 signaling pathway. We also identified that Tongxinluo (TXL), a traditional Chinese medicine, has beneficial effects on the treatment of DN in DN patients and type 2 diabetic mice. However, it remained elusive whether TXL could ameliorate renal structure and function through suppression of intercellular transfer of TGF-ß1-containing exosomes from GECs to GMCs. In this study, we demonstrate that TXL can inhibit the secretion of TGF-ß1-containing exosomes from HGtreated GECs. Furthermore, exosomes produced by HG induced-GECs treated with TXL cannot trigger GMC activation, proliferation and extracellular matrix (ECM) overproduction both in vitro and in vivo. These results suggest that TXL can prevent the transfer of TGF-ß1 from GECs to GMCs via exosomes, which may be one of the mechanisms of TXL in the treatment of DN. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
