التفاصيل البيبلوغرافية
| العنوان: |
Pathogenic role of glycan-specific IgG antibodies in IgA nephropathy. |
| المؤلفون: |
Yan-feng Zhao, Li Zhu, Li-jun Liu, Su-fang Shi, Ji-cheng Lv, Hong Zhang, Zhao, Yan-Feng, Zhu, Li, Liu, Li-Jun, Shi, Su-Fang, Lv, Ji-Cheng, Zhang, Hong |
| المصدر: |
BMC Nephrology; 9/29/2017, Vol. 18, p1-6, 6p |
| مصطلحات موضوعية: |
GLYCANS, IMMUNOGLOBULIN G, IGA glomerulonephritis, CELL proliferation, GALACTOSE, ANIMALS, AUTOANTIBODIES, CATTLE, CELL culture, CELL physiology, GLOMERULONEPHRITIS, IMMUNOGLOBULINS, IMMUNOLOGICAL adjuvants, KIDNEY glomerulus, POLYSACCHARIDES, PHARMACODYNAMICS |
| مستخلص: |
Background: Accumulating evidences proved the important roles of circulating IgA1-containing immune complexes (cIgA1) in IgA nephropathy (IgAN). Galactose-deficient IgA1 (Gd-IgA1) and glycan-specific IgG antibody have been identified as major components in cIgA1. Before, Gd-IgA1 was reported as a vital factor in IgAN, partly via of its pathogenic role to induce mesangial cells activation. However, we still lack direct evidences to clarify the biological effect of glycan-specific IgG antibody in IgAN.Methods: In the present study, we enrolled 35 IgAN patients and 17 age- and sex-matched healthy controls. Using uniform aberrant glycosylated IgA1 molecules, and IgG from different individuals, we in vitro prepared IgG-ddIgA1 complexes, and compared the biological differences among these immune complexes regarding their proliferative and inflammatory effects on mesangial cells.Results: IgG-ddIgA1 complexes from both patients with IgA nephropathy (IgAN-IgG-dd-IgA1) and healthy controls (HC-IgG-dd-IgA1) could induce the proliferation of mesangial cells and up-regulate expression of MCP-1, IL-6 and CXCL1. The levels of mesangial cells proliferation induced by IgAN-IgG-dd-IgA1 were significantly higher than those induced by HC-IgG-dd-IgA1 (1.10 ± 0.05 vs. 1.03 ± 0.03; p < 0.001). However, the levels of secreted MCP-1, IL-6 and CXCL1 from mesangial cells challenged by IgAN-IgG-dd-IgA1 and HC-IgG-dd-IgA1 were comparable.Conclusions: We found that glycan-specific IgG antibodies derived from patients with IgAN had the biological effect to induce mesangial cells proliferation. Moreover, in the present study we also established a method for in vitro preparation of pathogenic IgG-ddIgA1 complexes, which could be applied in future studies exploring IgAN pathogenesis. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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