التفاصيل البيبلوغرافية
| العنوان: |
V1b vasopressin receptor trafficking and signaling: Role of arrestins, G proteins and Src kinase. |
| المؤلفون: |
Perkovska, Sanja, Méjean, Catherine, Ayoub, Mohammed Akli, Li, Juan, Hemery, Floriane, Corbani, Maithé, Laguette, Nadine, Ventura, Maria‐Angeles, Orcel, Hélène, Durroux, Thierry, Mouillac, Bernard, Mendre, Christiane |
| المصدر: |
Traffic; Jan2018, Vol. 19 Issue 1, p58-82, 25p |
| مصطلحات موضوعية: |
CELLULAR signal transduction, VASOPRESSIN, ARRESTINS, MOLECULAR biology, G protein coupled receptors |
| مستخلص: |
The signaling pathway of G protein-coupled receptors is strongly linked to their trafficking profile. Little is known about the molecular mechanisms involved in the vasopressin receptor V1b subtype (V1bR) trafficking and its impact on receptor signaling and regulation. For this purpose, we investigated the role of β-arrestins in receptor desensitization, internalization and recycling and attempted to dissect the V1bR-mediated MAP kinase pathway. Using MEF cells Knocked-out for β-arrestins 1 and 2, we demonstrated that both β-arrestins 1 and 2 play a fundamental role in internalization and recycling of V1bR with a rapid and transient V1bR-β-arrestin interaction in contrast to a slow and long-lasting β-arrestin recruitment of the V2 vasopressin receptor subtype (V2R). Using V1bR-V2R chimeras and V1bR C-terminus truncations, we demonstrated the critical role of the V1bR C-terminus in its interaction with β-arrestins thereby regulating the receptor internalization and recycling kinetics in a phosphorylation-independent manner. In parallel, V1bR MAP kinase activation was dependent on arrestins and Src-kinase but independent on G proteins. Interestingly, Src interacted with hV1bR at basal state and dissociated when receptor internalization occurred. Altogether, our data describe for the first time the trafficking profile and MAP kinase pathway of V1bR involving both arrestins and Src kinase family. [ABSTRACT FROM AUTHOR] |
|
Copyright of Traffic is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder