التفاصيل البيبلوغرافية
| العنوان: |
Isolation of DNA from Chicken (Gallus gallus domesticus Linnaeus, 1758) Feather with Lysis Buffer-Phenol Chloroform Isoamyl Alcohol Method (PCI) and Chelex Method. |
| المؤلفون: |
Ernanto, Aditya Rahman, Afifah, Desiana, Lesmana, Indra, Daryono, Budi Setiadi |
| المصدر: |
AIP Conference Proceedings; 2018, Vol. 2002 Issue 2, p1-5, 5p, 1 Diagram, 2 Graphs |
| مصطلحات موضوعية: |
NUCLEIC acid isolation methods, CHICKENS, PHENOL analysis, SPECTROPHOTOMETERS, FEATHERS, ALCOHOL analysis, PHYSIOLOGY |
| مستخلص: |
DNA extraction material can be obtained from the body part or body of a cell-containing organism. Feather is part of the cover body that contains cell in the base. The use of feathers as a sample of DNA isolation or DNA genome sources minimizes the stress on the chicken and simplifies sampling process of many birds. This study aimed to compare the concentration and purity of the DNA isolates from lysis buffer-Phenol Chloroform Isoamyl alcohol (PCI) and chelex methods. Steps of procedure were collecting feathers from layer chicken, cutting base part of feathers, weighing feathers in 1 mg, 2 mg, 3 mg, 4 mg, and 5 mg with three repetitions, DNA isolation with buffer lysis-PCI method and with chelex method, and DNA quantification by nanodrop spectrophotometer. The average concentration produced in the lysis buffer-PCI method were 4.1 ng · µL-1, 8.0 ng · µL-1, 7.0 ng · µL-1, 9.3 ng · µL-1, and 23.3 ng · µL-1 respectively, whereas in the chelex method were 166.2 ng · µL-1, 277.8 ng · µL-1, 301.0 ng · µL-1, 318.3 ng · µL-1, and 404.3 ng · µL-1 respectively. The average purity rate produced in the lysis buffer-PCI method was 1.868, 1.617, 1.787, 1.766, and 1.826 respectively while in the chelex method were 0.723, 0.723, 0.733, 0.750, and 0.766 respectively. The conclusions of this study were the concentration of isolate by chelex method higher than buffer lysis-PCI method, the purity ratio of buffer lysis-PCI method was higher than chelex method. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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