التفاصيل البيبلوغرافية
| العنوان: |
Simultaneous Determination of Eight Kinds of Gingerols in Zingiberis Rhizome Collected From Different Areas of China. |
| المؤلفون: |
Yun Wang, Jianhong Chen, Yutian Li, Puling Li, Iqbal, Javed, Ying Chen, Yinlian Ma, Cun Zhang |
| المصدر: |
Acta Chromatographica; 2018, Vol. 30 Issue 3, p164-168, 5p |
| مصطلحات موضوعية: |
GINGER, HIGH performance liquid chromatography, ACETONITRILE, FORMIC acid, HERBAL medicine, PHARMACOLOGY |
| مستخلص: |
A reliable and rapid high-performance liquid chromatography coupled with diode array detector method (HPLC-DAD) was established and validated to determine eight gingerol simultaneously in the rhizomes of Zingiber offcinale Rosc. The separation of eight compounds (4-hydroxy-3-methoxy-benzenebutanol,3,5-dihydroxy-1-(4-hydroxy-3-methoxyphenyl) decane, 3,5-dihydroxy-1-(3,4-dimethoxyphenyl) decane, 6-gingerol, 8-gingerol, 6-shogaol, 5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-1,4-decadien-3-one, and 10-gingerol) were performed on an Agilent TC(2) C18 (250 mm × 4.6 mm, 5 μm) at 30 °C using acetonitrile (A) and 1% formic acid aqueous solution (B) as the mobile phase with gradient elution (0-10 min, 20%-35% A; 10-28 min, 35%-55% A; 28-35 min, 55%-60% A; 35-55 min, 60%-70% A; 55.01-60 min, 100%-100% A). The detection wavelength was set at 280 nm, and the flow rate was 0.8 mL/min. Validation of the analytical method was performed by linearity, precision, and accuracy test. All compounds were quantified with good linear calibration curves (coefficient of determination R2, >0.9999). The method showed good precision with overall coefficients of variation between 0.56% and 0.84%. The range of recovery was from 95.50% to 104.14% for the analytes. This method was successfully applied to quantify eight gingerols in Z. offcinale Rosc from different regions in China, so it can provide quality assessment for this medicine. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
