التفاصيل البيبلوغرافية
| العنوان: |
Effects and mechanisms of Bushen Tiaojing Fang on the embryo implantation via STAT3 signaling pathway during implantation window in superovulated mouse. (English) |
| المؤلفون: |
Duan Yancang, Li Zhili, Liu Wei, Fu Tian, Yang Jiangxia, Wang Yahui, Zhang Yayao, Hao Renjuan, Yu Huiwen, Du Huilan, Xu Huazhou, Ma Huirong, Song Cuimiao |
| المصدر: |
Journal of Beijing University of Traditional Chinese Medicine; Aug2018, Vol. 41 Issue 8, p648-654, 7p |
| مستخلص: |
Objective To observe the effects of Bushen Tiaojing Fang (BSTJF, kidney-tonifying menstruation-regulating formula) on endometrium of controlled ovarian hyperstimulated (COH) mice through signal transducer and activator of transcription 3 (STAT3), and to explore mechanisms of BSTJF for improving embryo implantation. Methods Sixty Kunming mice were randomly divided into the model group, BSTJF group and normal group (n =20 in each group). On the fourth day of pregnancy, 10 mice from each group were sacrificed. The levels of serum estradiol (E2) and progesterone (P) were measured by using radioimmunoassay. The expressions of endometrial leukemia inhibitory factor (LIF), phosphorylated STAT3 (P-STAT3) protein were evaluated with Western blot assay; Leptin and leptin receptor (OB-R) were detected with immunohistochemistry. At eighth day when all mice were then sacrificed, the average number of blastocysts was counted. Results Compared with the normal group, serum E2 level in the model group was decreased (P <0.05), and P was increased (P <0.01); the contents of LIF and P-STAT3 protein in the endometrium decreased (P <0.01); the expressions of Leptin and OB-R in epithelial cells decreased (P <0.01); the average number of implanted embryos was also decreased (P < 0.01). Compared with the model group, serum P decreased in the BSTJF group (P <0.05); the contents of LIF and P-STAT3 protein in endometrium increased (P <0.01), the expression of Leptin protein in endometrial epithelial cells increased (P < 0.05). The average number of blastocysts was increased (P<0.01). Conclusion BSTJF can improve the embryo implantation of COH mice. Its mechanism might be related to regulation of relative proteins in STAT3 signal pathway in the endometrium during im plantation window. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
