التفاصيل البيبلوغرافية
| العنوان: |
Myosin Heavy Chain Composition of Rat Middle Ear Muscles. |
| المؤلفون: |
Hak Hyun Jung, Seung Hoon Han, Soon Yuhl Nam, Young Ho Kim, Jeung Lim Kim |
| المصدر: |
Acta Oto-Laryngologica; Jun2004, Vol. 124 Issue 5, p569-573, 5p |
| مصطلحات موضوعية: |
BRANCHIAL cleft fistula, TYMPANIC membrane, MIDDLE ear, TYMPANIC plexus, MYOSIN, MESSENGER RNA, MUSCLE contraction |
| مستخلص: |
Objective --To quantitatively analyze myosin heavy chain (MHC) mRNA composition in two rat middle ear muscles (the tensor tympani and stapedius) using competitive polymerase chain reaction (PCR). Material and Methods --An exogenous template, including oligonucleotide sequences specific for the seven rat MHCs (2A, 2B, 2X, 2L/EOM, embryonic, neonatal and β-cardiac) as well as β-actin, was constructed and used as the competitor. Results --The tensor tympani and stapedius contained all MHC isoforms except 2L. The tensor tympani contained approximately equal proportions of 2X (40.4%±6.5%) and 2A (34.0%±1.3%) MHCs, with a smaller percentage of 2B (16.6%±1.5%) and neonatal (7.5%±0.6%) MHCs, while β-cardiac and embryonic MHCs were minimally expressed. The stapedius contained predominantly 2X (58.0%±4.2%) and 2A (32.3%±6.7%) MHCs, with a smaller percentage of 2B (7.4%±0.2%) and β-cardiac (1.9%±0.1%) MHCs. Neonatal and embryonic MHCs were detected at very low levels. Conclusion --These results suggest that two middle ear muscles, which are mainly composed of two fast-twitching myosins (2X and 2A MHCs), contract fast and are fatigue-resistant. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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