التفاصيل البيبلوغرافية
| العنوان: |
Bioassay for detection of methotrexate in serum. |
| المؤلفون: |
de Lathoulder, S., Gerards, A. H., de Groot, E. R., Valkhof, M .G., Dijkmans, B. A. C., Aarden, L .A., de Lathouder, S |
| المصدر: |
Scandinavian Journal of Rheumatology; May2004, Vol. 33 Issue 3, p167-173, 7p |
| مصطلحات موضوعية: |
METHOTREXATE, IMMUNOSUPPRESSIVE agents, PHARMACODYNAMICS, BIOACTIVE compounds, SERUM, FOLIC acid |
| مستخلص: |
Objective: A bioassay is developed for the measurement of methotrexate (MTX) in serum.Methods: The assay is based on MTX inhibition of the proliferation of hypoxanthine-guanosine phosphoribosyl transferase (HGPRT) negative mouse B-cells (B9.H). HGPRT negative cells cannot use the salvage pathway of nucleotide synthesis to overcome inhibition by MTX.Results: When B9.H cells are cultured with serial dilutions of serum, inhibition of proliferation is a measure of the amount of MTX in the serum. Circulating folates do not interfere with the assay.Conclusion: This simple assay can detect low concentrations of MTX in serum: it is therefore useful for following the pharmacodynamics of functional MTX after low-dose MTX treatment. [ABSTRACT FROM AUTHOR] |
|
Copyright of Scandinavian Journal of Rheumatology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
PDF full text from Publisher