التفاصيل البيبلوغرافية
العنوان: |
Bioassay for detection of methotrexate in serum. |
المؤلفون: |
de Lathoulder, S., Gerards, A. H., de Groot, E. R., Valkhof, M .G., Dijkmans, B. A. C., Aarden, L .A., de Lathouder, S |
المصدر: |
Scandinavian Journal of Rheumatology; May2004, Vol. 33 Issue 3, p167-173, 7p |
مصطلحات موضوعية: |
METHOTREXATE, IMMUNOSUPPRESSIVE agents, PHARMACODYNAMICS, BIOACTIVE compounds, SERUM, FOLIC acid |
مستخلص: |
Objective: A bioassay is developed for the measurement of methotrexate (MTX) in serum.Methods: The assay is based on MTX inhibition of the proliferation of hypoxanthine-guanosine phosphoribosyl transferase (HGPRT) negative mouse B-cells (B9.H). HGPRT negative cells cannot use the salvage pathway of nucleotide synthesis to overcome inhibition by MTX.Results: When B9.H cells are cultured with serial dilutions of serum, inhibition of proliferation is a measure of the amount of MTX in the serum. Circulating folates do not interfere with the assay.Conclusion: This simple assay can detect low concentrations of MTX in serum: it is therefore useful for following the pharmacodynamics of functional MTX after low-dose MTX treatment. [ABSTRACT FROM AUTHOR] |
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قاعدة البيانات: |
Complementary Index |