التفاصيل البيبلوغرافية
| العنوان: |
Accessory cell and HLA compatibility requirements for the generation of specific in-vitro antibody responses to influenza virus by human blood lymphocytes. |
| المؤلفون: |
Mitchell, D. M., Beverley, P. C. L., Boyle, Donna G., Winger, L. A., Cakkard, R. E. |
| المصدر: |
Immunology; Oct83, Vol. 50 Issue 2, p239-250, 12p |
| مصطلحات موضوعية: |
CELLS, RESPIRATORY infections, VIRUS diseases, INFLUENZA viruses, LEUCOCYTES, IMMUNOGLOBULINS, INFLUENZA, VIRUSES, COLLOIDS, MONOCLONAL antibodies |
| مستخلص: |
The role of antigen presenting cells in specific in-vitro antibody responses to influenza virus by human lymphocytes was investigated. Demonstration of a requirement for accessory cells necessitated vigorous depletion procedures. Accessory cells were removed by passage of mononuclear cells through a Sephadex 010 column followed by separation of cells bearing antigens defined by the monoclonal antibodies OKMI and UCHMI by fluorescence-activated cell sorting or panning. Peripheral blood mononuclear cells treated in this way were unable to respond to influenza virus by antibody production in either round-bottom or flat-bottom culture wells. The response could be reconstituted by either irradiated plastic-adherent cells or 010 non- adherent OKMI/UCHM1 positive cells in both autologous or allogeneic (HLA-DR different) combinations. The absence of HLA restriction was not due to non-specific allogeneic effects and was confirmed using irradiated, antigen-pulsed accessory cells which were extensively washed, incubated overnight and washed again to remove free antigen. These data suggest that the normal T-cell repertoire contains I helper cells specific for antigen plus self and antigen plus allo MHC. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
