التفاصيل البيبلوغرافية
| العنوان: |
Biosynthesis of 32 P-labelled hydroxocobalamin and a study of its behaviour in rats. |
| المؤلفون: |
R. Gräsbeck, Ebara, S., Aaltonen, J., Pinnioja, S. |
| المصدر: |
Scandinavian Journal of Clinical & Laboratory Investigation; Sep2004, Vol. 64 Issue 5, p451-456, 6p |
| مصطلحات موضوعية: |
BIOSYNTHESIS, VITAMIN B12, COBALT, PHOSPHORUS, PROPIONIBACTERIUM, LABORATORY rats |
| مستخلص: |
Using Propionibacterium freudenreichii and 32 P-ATP, batches of 32 P-labelled cobalamin (Cbl) were biosynthesized with a maximum specific activity of 61 μCi/mg, i.e. about 100 times higher than previously reported. Pharmacological doses mixed with 57 Co-Cbl were injected subcutaneously in the form of hydroxo-Cbl into rats subsequently killed 5-20 days later. The two labelled Cbls were distributed in approximately the same way, the highest concentration being found in kidney (typical for rats) and about one-fifth of that in liver. These findings tallied with previous observations with radioactive cyano-Cbl and microbiological assay. In all injected rats, the 57 Co/ 32 P ratio was lower in liver than in kidney. Drugs eradicating the intestinal flora had no influence. In rats receiving the vitamin orally, the ratio was higher in liver than in kidney. All of our findings could be due to formation of a cobinamide-like compound lacking phosphorus. It is concluded that we have produced radiophosphorus-labelled Cbl that enables studies in vivo . [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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