التفاصيل البيبلوغرافية
| العنوان: |
Over-Expression of IS Aba1 -Linked Intrinsic and Exogenously Acquired OXA Type Carbapenem-Hydrolyzing-Class D-ß-Lactamase-Encoding Genes Is Key Mechanism Underlying Carbapenem Resistance in Acinetobacter baumannii. |
| المؤلفون: |
Wong, Marcus Ho-yin, Chan, Bill Kwan-wai, Chan, Edward Wai-chi, Chen, Sheng |
| المصدر: |
Frontiers in Microbiology; 12/4/2019, Vol. 10, p1-9, 9p |
| مصطلحات موضوعية: |
ACINETOBACTER baumannii, ENZYME kinetics, ENZYME analysis, GENE expression, GENES, NUCLEOTIDE sequencing |
| مصطلحات جغرافية: |
HENAN Sheng (China), HONG Kong (China) |
| مستخلص: |
Acinetobacter baumannii is an important clinical pathogen which often causes fatal infections among seriously ill patients. Treatment options for managing infections caused by this organism have become limited as a result of emergence of carbapenem resistant strains. In the current study, whole genome sequencing, gene expression studies and enzyme kinetics analyses were performed to investigate the underlying carbapenem resistance mechanisms in fourteen clinical A. baumannii strains isolated from two hospitals, one each in Hong Kong and Henan Province, People's Republic of China. A large majority of the A. baumannii strains (11/14) were found to belong to the International Clone II (IC-II), among which six were ST208. Twelve of these strains were carbapenem resistant and found to either harbor bla OXA– 23/ bla OXA– 72, or exhibit over-expression of the bla OXA– 51 gene upon IS Aba1 insertion. Enzymatic assay confirmed that the OXA variants, including those of bla OXA –51, exhibited strong carbapenem-degrading activities. In terms of other intrinsic mechanisms, a weak correlation was observed between reduced production of outer membrane porin CarO/expression resistance-nodulation-division (RND) efflux AdeB and phenotypic resistance. This finding implied that over-production of carbapenem-hydrolyzing-class D-ß-lactamases (CHDLs), including the intrinsic bla OXA– 51 gene and the acquired bla OXA– 23 and bla OXA– 24 elements, is the key mechanism of carbapenem resistance in A. baumannii. This view is confirmed by testing the effect of NaCl, a known bla OXA inhibitor, which was found to cause reduction in carbapenem MIC by twofolds to eightfolds, suggesting that inhibiting OXA type carbapenemases represents the most effective strategy to control phenotypic carbapenem resistance in A. baumannii. [ABSTRACT FROM AUTHOR] |
|
Copyright of Frontiers in Microbiology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder