التفاصيل البيبلوغرافية
| العنوان: |
The role of CD4 molecules in the induction phase of contact hypersensitivity cytokine profiles in the skin and lymph nodes. |
| المؤلفون: |
Fujisawa, H., Kondo, S., Wang, B., Shivji, G. M., Sauder, D. N. |
| المصدر: |
Immunology; Oct96, Vol. 89 Issue 2, p250-255, 6p |
| مصطلحات موضوعية: |
CONTACT dermatitis, CELL membranes, GLYCOPROTEINS, SKIN, CYTOKINES, MESSENGER RNA, POLYMERASE chain reaction, IMMUNOLOGY |
| مستخلص: |
We have previously demonstrated that CD4 gene-targeted mutant mice (CD4- mice) demonstrate hyporesposiveness in contact hypersensitivity (CHS) suggesting that CD4 molecules are required for optimal induction of CHS. In the present study, we wished to examine the mechanisms of this hyporesponsiveness, in particular, we examined whether cytokines were altered in the skin and lymph nodes of CD4- mice following exposure to the contact allergen dinitrofluorobenzene (DNFB). Cytokine mRNA in the ear skin and draining lymph nodes (DLN) were examined by reverse transcription-polymerase chain reacion (RT- PCR) at various times after sensitization. Skin cytokine patterns revealed that in normal mice, interleukin (IL)-2, interferon (IFN)-γ and turnout necrosis factor (TNF)-α mRNA levels increased at 12 hr sensitization, whereas these cytokines were below the level of detection in CD4- mice. In the DLN of normal mice following the hapten application, sequential upregulation of cytokine mRNA including IL-1α, IL-lβ, IL-2, IL-4, IL-10, IFN-γ and TNF-α was found. No change was seen for IL-lα IL-1β IL-10 and TNF-α and IL-2, IL-4 and IFN-γ mRNA levels were below the level of detection in DLN from CD4 mice following the hapten application. However, IL-1β, IL-2 and TNF-α mRNA levels of lymph node cells from CD4- mice could be upregulated by phorbol myristate acetate in vitro. Flow cytometry study has revealed that the number of Langeshans' cells (LC) in DLN of CD4- mice was similar to that of normal mice, thus, inferring that the alterations of cytokine milieu in the ear skin did not have a significant effect on LC migration to DLN. These results suggest that CD4 molecules are crucial for the induction of certain cytokines in the skin and in inducing sequential cytokine signals in DLN required for optimal development of CHS, but that these changes in cytokines do not effect LC migration. [ABSTRACT FROM AUTHOR] |
|
Copyright of Immunology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder