التفاصيل البيبلوغرافية
| العنوان: |
K6-linked SUMOylation of BAF regulates nuclear integrity and DNA replication in mammalian cells. |
| المؤلفون: |
Qiaoyu Lin, Bin Yu, Xiangyang Wang, Shicong Zhu, Gan Zhao, Mingkang Jia, Fan Huang, Nan Xu, He Ren, Qing Jiang, Chuanmao Zhang |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America; 5/12/2020, Vol. 117 Issue 19, p1-10, 10p |
| مصطلحات موضوعية: |
DNA replication, NUCLEAR DNA, PROLIFERATING cell nuclear antigen |
| مصطلحات جغرافية: |
PAPHOS (Cyprus) |
| مستخلص: |
Barrier-to-autointegration factor (BAF) is a highly conserved protein in metazoans that has multiple functions during the cell cycle. We found that BAF is SUMOylated at K6, and that this modification is essential for its nuclear localization and function, including nuclear integrity maintenance and DNA replication. K6-linked SUMOylation of BAF promotes binding and interaction with lamin A/C to regulate nuclear integrity. K6-linked SUMOylation of BAF also supports BAF binding to DNA and proliferating cell nuclear antigen and regulates DNA replication. SENP1 and SENP2 catalyze the de-SUMOylation of BAF at K6. Disrupting the SUMOylation and de-SUMOylation cycle of BAF at K6 not only disturbs nuclear integrity, but also induces DNA replication failure. Taken together, our findings demonstrate that SUMOylation at K6 is an important regulatory mechanism that governs the nuclear functions of BAF in mammalian cells. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
