التفاصيل البيبلوغرافية
| العنوان: |
Screening for the human neutrophil antigen-2 deficiency by a multiplex one-tube polymerase chain reaction assay. |
| المؤلفون: |
Jiwu Gong, Meiying Rao, Zhonghua Jia, Na Ma, Lifang Sun, Xiaonan Yu, Feng Chen, Jue Xie, Hongwei Gong, Qiang Fu, Chen Cao, Fangfang Chen, Tongmao Zhao |
| المصدر: |
ISBT Science Series; May2020, Vol. 15 Issue 2, p286-291, 6p |
| مستخلص: |
Objectives The aim of this study was to develop a rapid method for human neutrophil antigen-2 (HNA-2) genotyping and use it for fast screening for the HNA- 2 deficiency phenotype in the Chinese population. Background HNA-2 is encoded by the CD177 gene, and a single nucleotide polymorphism (SNP) at cDNA position 787 (CD177*787 A > T) creates a stop codon, which is responsible for the HNA-2 deficiency phenotype. Recently, the SNP c.787A > T has been demonstrated to be the primary genetic mechanism for HNA-2 deficiency. Methods This study focused on determination of CD177 SNP c.787A > T. We designed a set of allele- and sequence-specific primers (SSP), which were used to develop a one-tube polymerase chain reaction (PCR) assay for HNA-2 genotyping. The HNA-2 genotype of a total of 2185 Chinese blood donors was determined. Results The validation of this HNA-2 genotyping assay was confirmed by sequencing analysis. The HNA-2 two alleles, CD177*787A and 787T, can be simultaneously determined in a single tube PCR. Thirteen individuals with the CD177*787AT genotype were detected in this study. The CD177*787TT homozygous genotype was absent, suggesting that HNA-2 deficiency is rare in the Chinese population. Conclusions The validated assay described in this study is a rapid and reliable method for HNA-2 genotyping, which may be used for fast screening for HNA-2 deficiency. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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