التفاصيل البيبلوغرافية
| العنوان: |
Genome-wide RNA interference screening reveals a COPI-MAP2K3 pathway required for YAP regulation. |
| المؤلفون: |
Yong Joon Kim, Eunji Jung, Eunbie Shin, Sin-Hyoung Hong, Hui Su Jeong, Gayeong Hur, Hye Yun Jeong, Seung-Hyo Lee, Ji Eun Lee, Gun-Hwa Kim, Joon Kim |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America; 8/18/2020, Vol. 117 Issue 33, p19994-20003, 10p |
| مصطلحات موضوعية: |
RNA interference, ENDOPLASMIC reticulum, CANCER cells |
| مستخلص: |
The transcriptional regulator YAP, which plays important roles in the development, regeneration, and tumorigenesis, is activated when released from inhibition by the Hippo kinase cascade. The regulatory mechanism of YAP in Hippo-low contexts is poorly understood. Here, we performed a genome-wide RNA interference screen to identify genes whose loss of function in a Hippo-null background affects YAP activity. We discovered that the coatomer protein complex I (COPI) is required for YAP nuclear enrichment and that COPI dependency of YAP confers an intrinsic vulnerability to COPI disruption in YAP-driven cancer cells. We identified MAP2K3 as a YAP regulator involved in inhibitory YAP phosphorylation induced by COPI subunit depletion. The endoplasmic reticulum stress response pathway activated by COPI malfunction appears to connect COPI and MAP2K3. In addition, we provide evidence that YAP inhibition by COPI disruption may contribute to transcriptional upregulation of PTGS2 and proinflammatory cytokines. Our study offers a resource for investigating Hippo-independent YAP regulation as a therapeutic target for cancers and suggests a link between YAP and COPI-associated inflammatory diseases. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
