التفاصيل البيبلوغرافية
| العنوان: |
Distinct Phenotypes of Islet Antigen-Specific CD4+ T Cells Among the 3 Subtypes of Type 1 Diabetes. |
| المؤلفون: |
Daisuke Chujo, Akitsu Kawabe, Maya Matsushita, Nobuyuki Takahashi, Chiharu Tsutsumi, Fumitaka Haseda, Akihisa Imagawa, Toshiaki Hanafusa, Kohjiro Ueki, Hiroshi Kajio, Kunimasa Yagi, Kazuyuki Tobe, Masayuki Shimoda, Chujo, Daisuke, Kawabe, Akitsu, Matsushita, Maya, Takahashi, Nobuyuki, Tsutsumi, Chiharu, Haseda, Fumitaka, Imagawa, Akihisa |
| المصدر: |
Journal of Clinical Endocrinology & Metabolism; Oct2020, Vol. 105 Issue 10, p1-11, 11p |
| مصطلحات موضوعية: |
TYPE 1 diabetes, T cells, GRANULOCYTES, ISLANDS of Langerhans, GLUTAMATE decarboxylase, TH1 cells, SUPPRESSOR cells |
| مستخلص: |
Context: Type 1 diabetes (T1D) is classified into 3 subtypes: acute-onset (AT1D), slowly progressive (SP1D), and fulminant (FT1D). The differences in the type of cellular autoimmunity within each subtype remain largely undetermined.Objective: To determine the type and frequency of islet antigen-specific CD4+ T cells in each subtype of T1D.Participants: Twenty patients with AT1D, 17 with SP1D, 18 with FT1D, and 17 persons without diabetes (ND).Methods: We performed an integrated assay to determine cellular immune responses and T-cell repertoires specific for islet antigens. This assay included an ex vivo assay involving a 48-hour stimulation of peripheral blood mononuclear cells with antigen peptides and an expansion assay involving intracytoplasmic cytokine analysis.Results: The results of the ex vivo assay indicated that glutamic acid decarboxylase 65 (GAD65)-specific interleukin-6 and interferon-inducible protein-10 (IP-10) responses and preproinsulin (PPI)-specific IP-10 responses were significantly upregulated in AT1D compared with those of ND. Furthermore, GAD65- and PPI-specific granulocyte colony-stimulating factor responses were significantly upregulated in FT1D. Expansion assay revealed that GAD65- and PPI-specific CD4+ T cells were skewed toward a type 1 helper T (Th1)- cell phenotype in AT1D, whereas GAD65-specific Th2 cells were prevalent in SP1D. GAD65-specific Th1 cells were more abundant in SP1D with human leukocyte antigen-DR9 than in SP1D without DR9. FT1D displayed significantly less type 1 regulatory T (Tr1) cells specific for all 4 antigens than ND.Conclusions: The phenotypes of islet antigen-specific CD4+ T cells differed among the three T1D subtypes. These distinct T-cell phenotypes may be associated with the manner of progressive β-cell destruction. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
