التفاصيل البيبلوغرافية
| العنوان: |
Molecular Dynamics Simulations Reveal Novel 12-Mer Peptide Derived From CPE30 of Clostridium Perfringens Targeting M Cell. |
| المؤلفون: |
Kien-Quang Huynh, Vertika Gautam, Byeol-Hee Cho, Yong-Suk Jang, Vannajan Sanghiran Lee, Hieu Tran-Van |
| المصدر: |
International Journal of Pharmaceutical Research & Allied Sciences; 2020, Vol. 9 Issue 4, p1-10, 10p |
| مصطلحات موضوعية: |
CLOSTRIDIUM perfringens, MOLECULAR dynamics, ORAL vaccines, CELL receptors, MOLECULAR docking, M cells |
| مستخلص: |
Oral vaccine has been become the most effective strategy in the fight against intestinal infections because of its economy, ease, and capability on inducing responses not only systemic but also local, especially in mucosal compartments. The major obstruction to potent vaccine development is antigen dispersion and tolerance. To overcome this hindrance, we aimed to target M cells, which are the main sentinel gateway for taking up luminal antigens and initiating specific mucosal responses. In this study, 12-mer peptide (CPE12) derived from the Cterminal of 30 amino acids of Clostridium perfringens (CPE30) was predicted to be a good binder using molecular docking and molecular dynamics simulations. To confirm the interaction between CPE12 and M cell receptor, the coding gene for CPE12 was cloned and expressed, CPE12 was then purified, and evaluated ex vivo afterwards. As a result, ex vivo assessment on murine M cells demonstrated that CPE12 had lower binding on murine M cells surface comparing to CPE30. These present results suggest that CPE12 could be a competent candidate peptide for oral vaccine development. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
