التفاصيل البيبلوغرافية
| العنوان: |
In vitro effects of Type I interferons (IFNτ and IFNα) on bovine hepatocytes cultured with or without Kupffer cells. |
| المؤلفون: |
Endriß, Kai Josef, Meyerholz, Marie Margarete, Fischbach, Teresa, Brimmers, Lutz, Pfarrer, Christiane, Marth, Christina Deborah, Schmicke, Marion |
| المصدر: |
Reproduction, Fertility & Development; 2021, Vol. 33 Issue 4, p305-317, 13p |
| مصطلحات موضوعية: |
TYPE I interferons, KUPFFER cells, SOMATOMEDIN C, LIVER cells, G proteins, GUANOSINE triphosphate, CHEMOKINES |
| مستخلص: |
In cattle, maternal recognition of early pregnancy depends on the effects of the embryonic signal interferon (IFN)-τ. IFN-stimulated genes have been upregulated in the maternal liver during early pregnancy. In this study, primary hepatocyte cell culture models were evaluated for their suitability to test Type I IFN effects in vitro. The expression of target genes (interferon-stimulated gene 15 (ISG-15), interferon-induced GTP-binding protein (MX-1), C-X-C motif chemokine 10 (CXCL-10), CXCL-5 , insulin-like growth factor 1 (IGF-1), IGF binding protein 2 (IGFBP-2)) was measured using reverse transcription–quantitative polymerase chain reaction in hepatocytes from monoculture or in indirect coculture with Kupffer cells (HKCid) on Days 1, 2, 3 and 4 of culture (n = 21 donor cows). Gene expression was also measured on Day 4 after challenging the cultures with recombinant IFNτ, IFNα, progesterone (P4), IFNτ + IFNα or IFNτ + P4 for 6 h. A significant increase in the mRNA expression of target genes in hepatocytes was shown in response to stimulation with IFNτ. The Kupffer cells in coculture did not influence the effects of IFNτ in hepatocytes. In conclusion, primary bovine hepatocyte cultures are suitable for stimulation experiments with Type I IFNs and as an extrauterine model for embryo–maternal communication. The proposed endocrine action of IFNτ in the liver may affect maternal metabolism and immune function in the liver. In cattle farming, embryo mortality has increased in recent years. To understand this problem, IFNτ is of enormous importance. The present study investigated the effects of IFNτ, other Type I IFNs and progesterone on hepatocytes. The results demonstrate that IFNτ induces the expression of different genes, resulting in a systemic effect. Therefore, this study forms the basis for further investigations of early embryo mortality in cows. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
