التفاصيل البيبلوغرافية
| العنوان: |
Radiological and Pathological Findings in a Minormismatch Mouse Orthotopic Lung Transplant Model under Immunosuppression. |
| المؤلفون: |
Nakagiri, T., Ahrens, L., Lienenklaus, S., Knöfel, A. K., Jonigk, D., Madrahimov, N., Jannson, K., Haverich, A., Warnecke, G. |
| المصدر: |
International Journal of Organ Transplantation Medicine; 2021, Vol. 12 Issue 1, p1-11, 11p |
| مصطلحات موضوعية: |
COMPUTED tomography, LUNG transplantation, GRAFT rejection, MICE, IMMUNOSUPPRESSION |
| مستخلص: |
Background: Mouse orthotopic lung transplantation (MOLTx) models are extremely useful. However, there are only few studies on non-invasive monitoring methods for lung rejection in these models. Additionally, a model of chronic rejection has so far been difficult to reproduce consistently with MOLTx. Objective: To determine if CT scan of the lung graft can be considered a useful noninvasive monitoring method of evaluation of graft rejection in an animal lung transplantation model. Methods: Left MOLTx was performed from B10 donors into B6 recipients (from B6 donors for syngeneic controls). Following transplantation, 3 different doses of cyclosporine--5, 15, and 25 mg/kg daily--were administered in the first week to suppress acute rejection. Positive controls did not receive cyclosporine. 8 weeks after transplantation, CT and histological rejection grading were performed. Results: The negative controls did not show any inflammation. Positive controls revealed moderate acute rejection (A3). A3 was also detected in the 5-mg/kg group (100%). The 15-mg/kg group (n=7) demonstrated A3 in 4 mice and mild acute rejection (A2) in the remaining 3 mice. In this group, 4 mice had bronchiolitis obliterans (BO; C1, 57%). The 25-mg/kg group (n=3) showed A3 changes in 1 and A2 in 2 mice. On CT scan, lungs without BO (C0) had radiodensities of -278.1±110.7 Hounsfield units (HU). C1 lungs had ground-glass opacity or atelectasis with -83.4±46.8 HU (C0 vs. C1, p<0.001). On grouping with A2 or A3 in C0, significant differences were detected: -375.3±41.2 vs. -185.0±38.4 HU (A2+C0 vs. A3+C0, respectively), p=0.01. Conclusion: BO can be modeled using this MOLTx model by administration of subtherapeutic doses of cyclosporine. CT scans are a valid tool for monitoring of rejection following MOLTx. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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