التفاصيل البيبلوغرافية
| العنوان: |
Effect of Low-Dose Irradiation on Human Dermal Stem Cells. |
| المؤلفون: |
Kumar B., Mohana, Rao, Shama, Vinutha E., Shetty, Jayaprakasha, Shetty, Nikhil, Rao K., Mallikarjuna, Sandeep, Ail, Shetty K., Jayarama |
| المصدر: |
Nitte University Journal of Health Science; 2018 Special Issue, p166-166, 1p |
| مصطلحات موضوعية: |
HUMAN stem cells, DOSE-response relationship (Radiation), CELL morphology, IRRADIATION, DNA repair, STEM cells |
| مستخلص: |
Background: Skin stem cells might be the major target to assess the ionising radiation effects. Recent evidences suggest that it may have significant effects on cellular functions. Because of their regenerative potential, dermal stem cells (DSCs) have been a subject of intense research, and they may provide an efficient means for treating radiation-induced tissue damage. Objective: The present study evaluated the effect of low dose irradiation on DSCs in terms of proliferation, potency and DNA fragmentation status. Methodology: Human DSCs were exposed to single doses of X-ray radiation 0, 0.5 and 2 Gy and assessed cell morphology, viability, proliferation, cell cycle status, senescence activity as well as the DNA fragmentation status. Results: DSCs showed a normal fibroblastic morphology, maintained a regular size, and were able to increase in number in 0.5 Gy group comparing to that of unirradiated DSCs. But irradiated DSCs at 2 Gy showed noticeable changes in morphology, viability and a decrease in proliferation gradually after day 6 of culture. The percentage of G0/G1 cells in DSCs in control was higher than the irradiated groups. However, senescence associated betagalactosidase assay revealed no presence of any senescent cells following 48 hrs post-irradiation. DNA fragmentation assay also showed no differences in the fragmented and intact DNA fractions between the control and irradiated DSCs. Conclusion: The findings showed that DSCs were resistant to low levels of ionising radiation with normal cellular characteristics and DNA repair capacity. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
