التفاصيل البيبلوغرافية
| العنوان: |
Radical Dehalogenation and Purine Nucleoside Phosphorylase E. coli : How Does an Admixture of 2′,3′-Anhydroinosine Hinder 2-fluoro-cordycepin Synthesis. |
| المؤلفون: |
Kayushin, Alexey L., Tokunova, Julia A., Fateev, Ilja V., Arnautova, Alexandra O., Berzina, Maria Ya., Paramonov, Alexander S., Lutonina, Olga I., Dorofeeva, Elena V., Antonov, Konstantin V., Esipov, Roman S., Mikhailopulo, Igor A., Miroshnikov, Anatoly I., Konstantinova, Irina D. |
| المصدر: |
Biomolecules (2218-273X); Apr2021, Vol. 11 Issue 4, p539-539, 1p |
| مصطلحات موضوعية: |
DEHALOGENATION, CHEMICAL precursors, CHEMICAL yield, CHEMICAL synthesis, MASS spectrometry |
| مستخلص: |
During the preparative synthesis of 2-fluorocordycepin from 2-fluoroadenosine and 3′-deoxyinosine catalyzed by E. coli purine nucleoside phosphorylase, a slowdown of the reaction and decrease of yield down to 5% were encountered. An unknown nucleoside was found in the reaction mixture and its structure was established. This nucleoside is formed from the admixture of 2′,3′-anhydroinosine, a byproduct in the preparation of 3-′deoxyinosine. Moreover, 2′,3′-anhydroinosine forms during radical dehalogenation of 9-(2′,5′-di-O-acetyl-3′-bromo- -3′-deoxyxylofuranosyl)hypoxanthine, a precursor of 3′-deoxyinosine in chemical synthesis. The products of 2′,3′-anhydroinosine hydrolysis inhibit the formation of 1-phospho-3-deoxyribose during the synthesis of 2-fluorocordycepin. The progress of 2′,3′-anhydroinosine hydrolysis was investigated. The reactions were performed in D2O instead of H2O; this allowed accumulating intermediate substances in sufficient quantities. Two intermediates were isolated and their structures were confirmed by mass and NMR spectroscopy. A mechanism of 2′,3′-anhydroinosine hydrolysis in D2O is fully determined for the first time. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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