التفاصيل البيبلوغرافية
| العنوان: |
Appropriate growth models to describe early growth of Kejobong goat based on Growth Hormone (GH) gene sequence analysis. |
| المؤلفون: |
Sutopo, S., Lestari, D. A., Oikawa, T., Konno, T., Purbowati, E., Setiaji, A., Kurnianto, E. |
| المصدر: |
Journal of the Indonesian Tropical Animal Agriculture; Jun2021, Vol. 46 Issue 2, p124-135, 12p |
| مصطلحات موضوعية: |
SOMATOTROPIN, SEQUENCE analysis, GOATS, BODY weight, GENES, GENETIC markers |
| Abstract (English): |
The objectives of this study were to reveal appropriate growth models describing early growth of Kejobong goat based on Growth Hormone (GH) gene sequence analysis. A total of 35 DNA samples and 1.960 records of quantitative traits of Kejobong goat were collected. The exon 3 of GH gene was amplified and was sequenced to determine the SNP. Body weight and body measurements of the goats were taken at 0-14 weeks of age. Four non-linear growth models were applied for analysis of growth to compare growth performance of different genotypes by Non-Linear Mixed Model. A non-synonymous mutation (g1170A→G) genotyped into GG, AG and AA was significantly associated with growth traits. Animals with heterozygous genotype AG showed higher growth traits than animals with homozygous genotype AA. Nonetheless, animals with homozygous genotype GG had the same growth traits with those animals with heterozygous genotype AG and homozygous genotype AA. The most fitted model for describing body weight was Von Bertalanffy model, while for describing wither height and hip height was Brody model. SNP at exon 3 of the GH gene can be used as genetic marker for improvement of growth traits of Kejobong goats. [ABSTRACT FROM AUTHOR] |
| Abstract (Indonesian): |
Tujuan penelitian yaitu untuk menemukan model pertumbuhan yang tepat dalam mendeskripsikan pertumbuhan awal kambing Kejobong berdasarkan analisis sekuen gen Growth Hormon (GH). Materi penelitian menggunakan 35 sampel DNA dan 1.960 catatan sifat kuantitatif kambing Kejobong. Sampel DNA diamplifikasi dan disekuensing untuk mengidentifikasi SNP yang terdapat pada gen GH ekson 3. Pengukuran dan penimbangan bobot badan dan ukuran tubuh dilakukan pada umur 0-14 minggu. Empat model pertumbuhan non-linier diaplikasikan untuk menganalisis pertumbuhan guna membandingkan performan pertumbuhan dari berbagai genotipe dengan menggunakan Non-Linear Mixed model. Mutasi non-sinonim (g1170A→G) pada gen GH ekson 3 yang membentuk genotipe GG, AG dan AA secara signifikan berasosiasi dengan sifat pertumbuhan. Kambing Kejobong bergenotipe heterozigot AG menunjukkan sifat pertumbuhan yang lebih tinggi dibandingkan dengan kambing Kejobong bergenotipe homozigot AA. Meskipun demikian, kambing Kejobong bergenotipe homozigot GG memiliki sifat pertumbuhan yang sama dengan kambing Kejobong bergenotipe heterozigot AG dan homozigot AA. Model pertumbuhan yang paling tepat untuk mendeskripsikan bobot badan kambing Kejobong adalah model Von Bertalanffy, sedangkan untuk menggambarkan tinggi badan dan tinggi pinggul adalah model Brody. SNP pada gen GH ekson 3 dapat digunakan sebagai penanda genetik untuk perbaikan sifat pertumbuhan kambing Kejobong. [ABSTRACT FROM AUTHOR] |
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