التفاصيل البيبلوغرافية
| العنوان: |
IPA1 Negatively Regulates Early Rice Seedling Development by Interfering with Starch Metabolism via the GA and WRKY Pathways. |
| المؤلفون: |
He, Yonggang, Zhu, Menghao, Li, Zhihui, Jiang, Shan, He, Zijun, Xu, Shuang, Chen, Xiangsong, Hu, Zhongli, Zhang, Zhihong |
| المصدر: |
International Journal of Molecular Sciences; Jun2021, Vol. 22 Issue 12, p6605, 1p |
| مصطلحات موضوعية: |
STARCH metabolism, GIBBERELLINS, RICE, SEEDLINGS, GERMINATION, SUGAR analysis, TRANSCRIPTION factors |
| مستخلص: |
Ideal Plant Architecture 1 (IPA1) encodes SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 14 (SPL14) with a pleiotropic effect on regulating rice development and biotic stress responses. To investigate the role of IPA1 in early seedling development, we developed a pair of IPA1/ipal-NILs and found that seed germination and early seedling growth were retarded in the ipa1-NIL. Analysis of the soluble sugar content, activity of amylase, and expression of the α-amylase genes revealed that the starch metabolism was weakened in the ipa1-NIL germinating seeds. Additionally, the content of bioactive gibberellin (GA) was significantly lower than that in the IPA1-NIL seeds at 48 h of imbibition. Meanwhile, the expression of GA synthesis-related gene OsGA20ox1 was downregulated, whereas the expression of GA inactivation-related genes was upregulated in ipa1-NIL seeds. In addition, the expression of OsWRKY51 and OsWRKY71 was significantly upregulated in ipa1-NIL seeds. Using transient dual-luciferase and yeast one-hybrid assays, IPA1 was found to directly activate the expression of OsWRKY51 and OsWRKY71, which would interfere with the binding affinity of GA-induced transcription factor OsGAMYB to inhibit the expression of α-amylase genes. In summary, our results suggest that IPA1 negatively regulates seed germination and early seedling growth by interfering with starch metabolism via the GA and WRKY pathways. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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