التفاصيل البيبلوغرافية
| العنوان: |
Na+, K+‐ATPase participates in the protective mechanism of rat cerebral ischemia‐reperfusion through the interaction with glutamate transporter‐1. |
| المؤلفون: |
Li, Lin‐Lin, Ke, Xue‐Ying, Jiang, Chen, Qin, Shi‐Qi, Liu, Yang‐Yang, Xian, Xiao‐Hui, Liu, Li‐Zhe, He, Jin‐Chen, Chen, Ya‐Meng, An, Hong‐Fei, Sun, Nan, Hu, Yue‐Hua, Wang, Yan, Zhang, Li‐Nan, Lu, Qi‐Yong |
| المصدر: |
Fundamental & Clinical Pharmacology; Oct2021, Vol. 35 Issue 5, p870-881, 12p |
| مصطلحات موضوعية: |
MYOCARDIAL reperfusion, ARTERIAL occlusions, GLUTAMIC acid, REPERFUSION injury, CEREBRAL infarction |
| مستخلص: |
Glutamate excitotoxicity in cerebral ischemia/reperfusion is an important cause of neurological damage. The aim of this study was to investigate the mechanism of Na+, K+‐ATPase (NKA) involved in l ow concentration of ouabain (Oua, activating NKA)‐induced protection of rat cerebral ischemia‐reperfusion injury. The 2,3,5‐triphenyltetrazolium chloride (TTC) staining and neurological deficit scores (NDS) were performed to evaluate rat cerebral injury degree respectively at 2 h, 6 h, 1 d and 3 d after reperfusion of middle cerebral artery occlusion (MCAO) 2 h in rats. NKA α1/α2 subunits and glutamate transporter‐1 (GLT‐1) protein expression were investigated by Western blotting. The cerebral infarct volume ratio were evidently decreased in Oua group vs MCAO/R group at 1 d and 3 d after reperfusion of 2 h MCAO in rats (*p ＜ 0.05). Moreover, NDS were not significantly different (p ＞ 0.05). NKA α1 was decreased at 6 h and 1 d after reperfusion of 2 h MCAO in rats, and was improved in Oua group. However, NKA α1 and α2 were increased at 3 d after reperfusion of 2 h MCAO in rats, and was decreased in Oua group. GLT‐1 was decreased at 6 h, 1 d and 3 d after reperfusion of 2 h MCAO in rats, and was improved in Oua group. These data indicated that l ow concentration of Oua could improve MCAO/R injury through probably changing NKA α1/α2 and GLT‐1 protein expression, then increasing GLT‐1 function and promoting Glu transport and absorption, which could be useful to determine potential therapeutic strategies for patients with stroke. Low concentration of Oua improved rat MCAO/R injury via NKA α1/α2 and GLT‐1. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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